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Inhibition of cathepsin D prevents free-radical-induced apoptosis in rat cardiomyocytes
Linköping University, Faculty of Health Sciences. Linköping University, Department of Neuroscience and Locomotion, Pathology. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Pathology and Clinical Genetics.
2000 (English)In: Archives of Biochemistry and Biophysics, ISSN 0003-9861, Vol. 373, no 2, 346-351 p.Article in journal (Refereed) Published
Abstract [en]

Apoptosis was inhibited in rat cardiomyocytes pretreated with the aspartic protease inhibitor pepstatin A and subsequently exposed to naphthazarin (5,8-dihydroxy-1,4-naphthoquinone). Cathepsin D was released from lysosomes to the cytosol upon exposure to naphthazarin, and the enzyme activity decreased simultaneously. Later, cathepsin D reappeared in granules of increased size, and enzyme activity was restored. Activation of caspase-3- like proteases was detected, and the number of cells showing apoptotic morphology increased with time. Pepstatin A pretreatment did not prevent release of cathepsin D from lysosomes but did significantly inhibit subsequent naphthazarin-induced caspase activation and apoptotic morphology. This suggests that cathepsin D exerts its apoptosis-stimulating effect upstream of caspase-3-like activation. (C) 2000 Academic Press.

Place, publisher, year, edition, pages
2000. Vol. 373, no 2, 346-351 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-27973DOI: 10.1006/abbi.1999.1567Local ID: 12733OAI: diva2:248524
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2011-01-14

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Öllinger, Karin
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ReferencesLink to record
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