Effects of pH on platelets release of growth factors in wound healing
2003 (English)Licentiate thesis, comprehensive summary (Other academic)
Wound healing is thought to involve a complex series of interactions between biochemical mediators, cells and extracellular matrix. Known to stimulate cellular movement, proliferation and biosynthetic activity, growth factors may play a significant role in stimulating wound repair. This thesis focuses on the early phase of wound healing. We established an experimental model which mimics the early proliferative stage and concentrated on a potentially interesting aspect of the wound environment pH as a modulator of the release of growth factors from platelets. The influence of platelet-rich plasma lysates on fibroblast proliferation at varying pH was studied in culture. We found that the concentration of platelet-derived growth factor in the different lysates was highest at pH 5.0. The concentration of transforming growth factor beta, however, was lower after incubation at pH 5.0 than at pH 7.1 and 7.6. Fibroblast proliferation and collagen production were enhanced by platelet lysates at pH 5.0, 7.1 and 7.6.We also studied the release of growth factors from platelet supernatants which were thrombin-activated at pH 7.4, then the pH was lowered to 5.0, remained the same or was raised to 8.5, or were thrombin-activated (or not) at pH 5.0, 7.4 and 8.5. Our results suggest that release of growth factors is dependent not only on platelet activation, but also on long-term incubation at 37°C and change in pH. ln summary, we found that pH may influence the activity of platelet proteins, resulting in fibroblast growth and promotion of wound healing.
Place, publisher, year, edition, pages
Linköping: Linköpings universitet , 2003. , 46 p.
Linköping Studies in Health Sciences. Thesis, ISSN 1100-6013 ; 59
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-28096Local ID: 12862ISBN: 91-7373-590-2OAI: oai:DiVA.org:liu-28096DiVA: diva2:248647
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