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Effects of pH on platelets release of growth factors in wound healing
Linköping University, Department of Clinical and Experimental Medicine, Experimental Pathology. Linköping University, Faculty of Health Sciences.
2003 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Wound healing is thought to involve a complex series of interactions between biochemical mediators, cells and extracellular matrix. Known to stimulate cellular movement, proliferation and biosynthetic activity, growth factors may play a significant role in stimulating wound repair. This thesis focuses on the early phase of wound healing. We established an experimental model which mimics the early proliferative stage and concentrated on a potentially interesting aspect of the wound environment pH as a modulator of the release of growth factors from platelets. The influence of platelet-rich plasma lysates on fibroblast proliferation at varying pH was studied in culture. We found that the concentration of platelet-derived growth factor in the different lysates was highest at pH 5.0. The concentration of transforming growth factor beta, however, was lower after incubation at pH 5.0 than at pH 7.1 and 7.6. Fibroblast proliferation and collagen production were enhanced by platelet lysates at pH 5.0, 7.1 and 7.6.We also studied the release of growth factors from platelet supernatants which were thrombin-activated at pH 7.4, then the pH was lowered to 5.0, remained the same or was raised to 8.5, or were thrombin-activated (or not) at pH 5.0, 7.4 and 8.5. Our results suggest that release of growth factors is dependent not only on platelet activation, but also on long-term incubation at 37°C and change in pH. ln summary, we found that pH may influence the activity of platelet proteins, resulting in fibroblast growth and promotion of wound healing.

Place, publisher, year, edition, pages
Linköping: Linköpings universitet , 2003. , 46 p.
Series
Linköping Studies in Health Sciences. Thesis, ISSN 1100-6013 ; 59
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-28096Local ID: 12862ISBN: 91-7373-590-2 OAI: oai:DiVA.org:liu-28096DiVA: diva2:248647
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2013-09-12
List of papers
1. Fibroblast proliferation due to exposure to a platelet concentrate in vitro is pH dependent
Open this publication in new window or tab >>Fibroblast proliferation due to exposure to a platelet concentrate in vitro is pH dependent
2002 (English)In: Wound Repair and Regeneration, ISSN 1067-1927, E-ISSN 1524-475X, Vol. 10, no 5, 336-340 p.Article in journal (Refereed) Published
Abstract [en]

The influence of platelet-rich plasma lysates on fibroblast proliferation was studied in culture. Cells were exposed to platelet lysates that had been preincubated at different pHs (5.0, 7.1, and 7.6). Proliferation was evaluated with the MTT assay and incorporation of [3H]thymidine into macromolecules, while type I collagen production was assayed by Western blotting. Enzyme-linked immunosorbent assays were used to determine platelet-derived growth factor and transforming growth factor-β concentrations. Platelets preincubated in an acidic environment (pH 5.0) induced the highest degree of fibroblast proliferation, and the concentration of platelet-derived growth factor in the different treated lysates was the highest at that particular pH. The concentration of transforming growth factor-β, however, was lower after incubation at pH 5.0 than at either pH 7.1 or 7.6. These findings may be relevant to normal wound healing in vivo and useful in the treatment of wounds and delayed healing processes.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-27830 (URN)10.1046/j.1524-475X.2002.10510.x (DOI)000178921600010 ()12587 (Local ID)12587 (Archive number)12587 (OAI)
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2013-09-12
2. Time- and pH-dependent release of PDGF and TGF-ß from platelets in vitro
Open this publication in new window or tab >>Time- and pH-dependent release of PDGF and TGF-ß from platelets in vitro
2003 (English)In: Platelets, ISSN 0953-7104, E-ISSN 1369-1635, Vol. 14, no 4, 233-237 p.Article in journal (Refereed) Published
Abstract [en]

We studied the spontaneous and thrombin-induced activation of platelets and their release of platelet-derived growth factor (PDGF) and transforming growth factor-β (TGF-β) at different pH values. Platelet activation was assayed with anti-bodies against P-selectin and performed in serum-free media. The release of PDGF and TGF-β was determined by ELISA after 15 min and 12 h. There was no activation at pH 5.0, while a time-dependent release of growth factors occurred at neutral and alkaline pH. The results suggest that release of growth factors is not only dependent on platelet activation but also on incubation time and pH. Although the used serum-free experimental situation is different from normal conditions for platelets in vivo, the findings of a late release of growth factors may, nevertheless, be relevant to wound healing.

National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-27831 (URN)10.1080/0953710031000118876 (DOI)000183567300005 ()12588 (Local ID)12588 (Archive number)12588 (OAI)
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2013-09-12

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