liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Lectin affinity capillary electrophoresis in glycoform analysis applying the partial filling technique
Show others and affiliations
2004 (English)In: Journal of chromatography. B, ISSN 1570-0232, Vol. 809, no 2, 323-329 p.Article in journal (Refereed) Published
Abstract [en]

The study of protein glycosylation and its significance in biological interactions is a field of growing interest. This work demonstrates a lectin-based separation of protein glycoforms of α1-acid glycoprotein (AGP or orosomucoid) with capillary electrophoresis. Glycoform analysis was performed with a "partial filling technique" with the lectin Concanavalin A (Con A) as affinity ligand. Con A separated human AGP into two peaks, the first peak included AGP glycoforms without biantennary glycans, and the second peak represented the fraction that had one or more biantennary glycans. The applicability of the method was demonstrated with the analysis of AGP from clinical samples and AGP treated with N-glycosidase F. The AGP separation was also used as a reporter system to estimate the dissociation constant (KD) between Con A and a competing sugar. © 2004 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
2004. Vol. 809, no 2, 323-329 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-28160DOI: 10.1016/j.jchromb.2004.06.042Local ID: 12975OAI: oai:DiVA.org:liu-28160DiVA: diva2:248711
Available from: 2009-10-08 Created: 2009-10-08 Last updated: 2011-01-12

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Påhlsson, Peter

Search in DiVA

By author/editor
Påhlsson, Peter
By organisation
Faculty of Health SciencesDivision of cell biology
In the same journal
Journal of chromatography. B
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 44 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf