Light microscopic assessment of the dermal inflammatory cell infiltrate in experimental inflammatory reactions
1999 (English)Licentiate thesis, comprehensive summary (Other academic)
In vivo experimental models have been used traditionally for the study of inflammatory contact reactions in the skin. The naked eye appearance and the histology of the skin reaction is in principle similar to that seen in man. More easily than in human experimentation, a cutaneous inflammatory reaction can, by the use of adequate group size, be studied histologically at many different time points. Plastic embedding and staining with May-GrUnewald Giemsa stains allow microscopic assessment of inflammatory cells infiltrating from the blood. Standardized, predetermined view-field selection and cellular criteria are necessary. Having previously studied the dermal infiltrate in contact reactions of delayed onset, the immediate Type I hypersensitivity reaction to ovalbumin and the non immunological immediate contact reaction to dimethyl sulfoxide (DMSO) were studied. Using the same experimental method, the effect of local application of the glucocorticosteroid, budesonide, on the delayed contact inflammatory reactions was also studied. The immediate Type I hypersensitivity reaction showed a dermal cellular infiltrate that was not uniformly distributed nor limited to the subepidermal area. The chronological study showed an influx of neutrophil and eosinophil granulocytes in the early phase and basophil granulocytes and mononuclear cells (chiefly lymphocytes) in the late phase. The non immunological immediate reaction to 100% DMSO showed the majority of the early infiltrating granulocytes to be basophils. One application of DMSO 12% gave no visible or microscopic reaction but repeated applications resulted in a delayed onset reaction with a predominant mononuclear cell infiltrate. Comparison of the two early reactions and the three previously studied reaction types showed differences and similarities at various time points in the dermal inflammatory cell infiltrate. Topically applied budesonide and its acetone vehicle showed anti inflammatory effects most pronounced on the toxic reaction to croton oil.
The present model illustrates differences in dermal inflammatory histological patterns in various common model reaction types. The findings are relevant for the discussion of pathogenetic mechanisms and constitute background information for continued clinical studies.
Place, publisher, year, edition, pages
Linköping: Linköping University , 1999. , 44 p.
Linköping Studies in Health Sciences. Thesis, ISSN 1100-6013 ; 43
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-28305Local ID: 13418ISBN: 91-7219-563-0OAI: oai:DiVA.org:liu-28305DiVA: diva2:249111
Papers, included in licentiate theses, are not registered and included in the posts from 1999 and earlier.2009-10-092009-10-092013-07-08