Clinical isolates of Staphylococcus aureus vary in ability to stimulate cytokine expression in human endothelial cells
2005 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, Vol. 61, no 1, 57-62 p.Article in journal (Refereed) Published
Human umbilical vein endothelial cells (HUVEC) were infected for 24 h with 18 well-characterized Staphylococcus aureus isolates, and the supernatants from infected HUVEC were analysed for interleukin (IL)-1β, tumour necrosis factor-alpha, IL-6, IL-8, IL-10, IL-12p70, growth-related oncogene (GRO)-α, granulocyte macrophage colony-stimulating factor (GM-CSF) and regulated upon activation, normal T cell expressed and secreted (RANTES) by immunoassay. All staphylococcal isolates induced the expression of IL-6, IL-8, GRO-α, GM-CSF and RANTES. The magnitude of cytokine expression varied between isolates. Staphylococcus aureus inducing high expression of one of these cytokines also showed simultaneous high expression of the other four, indicating a common mechanism for the ability of individual S. aureus to induce expression of these cytokines. No direct correlation between cytokine expression and adhesion of S. aureus to HUVEC was observed, indicating that bacterial properties besides adhesion contribute to the activation of HUVEC.
Place, publisher, year, edition, pages
2005. Vol. 61, no 1, 57-62 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-28869DOI: 10.1111/j.0300-9475.2005.01526.xLocal ID: 14063OAI: oai:DiVA.org:liu-28869DiVA: diva2:249681