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Acidic preparations of platelet concentrates release bone morphogenetic protein-2.
Linköping University, Department of Clinical and Experimental Medicine, Orthopaedics and Sports Medicine . Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Orthopaedic Centre, Department of Orthopaedics Linköping.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Chemistry.
Linköping University, Department of Clinical and Experimental Medicine, Orthopaedics and Sports Medicine . Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Orthopaedic Centre, Department of Orthopaedics Linköping.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Chemistry.
2008 (English)In: Acta orthopaedica, ISSN 1745-3682, Vol. 79, no 3, 433-437 p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND AND PURPOSE: Growth factors released from platelets have potent effects on fracture and wound healing. The acidic tide of wound healing, i.e. the pH within wounds and fractures, changes from acidic pH to neutral and alkaline pH as the healing process progresses. We investigated the influence of pH on lysed platelet concentrates regarding the release of growth factors. MATERIAL AND METHODS: Platelet concentrates free of leukocyte components were lysed and incubated in buffers with pH between 4.3 and 8.6. Bone morphogenetic protein-2 (BMP-2), platelet-derived growth factor (PDGF), transforming growth factor-beta(TGF-beta), and vascular endothelial growth factor (VEGF) were measured by quantitative enzyme-linked immunosorbent assays. RESULTS: PDGF, TGF-beta, and VEGF were present in all platelet preparations but the levels varied in a pH-dependent fashion. BMP-2 was only detected in the most acidic preparation (pH 4.3), which is interesting since BMP-2 has been reported to be an endogenous mediator of fracture repair and to be responsible for the initiation of fracture healing. INTERPRETATION: Our findings indicate that platelets release substantial amounts of BMP-2 only under conditions of low pH, the milieu associated with the critical initial stage of fracture healing.

Place, publisher, year, edition, pages
2008. Vol. 79, no 3, 433-437 p.
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Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-29162DOI: 10.1080/17453670710015364PubMedID: 18626808OAI: oai:DiVA.org:liu-29162DiVA: diva2:250182
Note
This is an electronic version of an article published in: Ola Wahlström, Cecilia Linder, Anders Kalén and Per Magnusson, Acidic preparations of platelet concentrates release bone morphogenetic protein-2., 2008, Acta orthopaedica, (79), 3, 433-437. http://dx.doi.org/10.1080/17453670710015364 Copyright: Taylor & Francis http://www.tandf.co.uk/journals/default.asp Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2009-10-09

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Wahlström, OlaLinder, CeciliaKalén, AndersMagnusson, Per

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