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The immunological effect of photopheresis in children with newly diagnosed type 1 diabetes
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Pediatrics.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Clinical Immunology. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Immunology and Transfusion Medicine.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Transfusion Medicine. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Immunology and Transfusion Medicine.
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2005 (English)In: Pediatric Research, ISSN 0031-3998, E-ISSN 1530-0447, Vol. 58, no 3, 459-466 p.Article in journal (Refereed) Published
Abstract [en]

Photopheresis has been claimed to have immune-modulating effects, but the mechanisms of action are unknown. This study investigated the immune effect of photopheresis in children with type 1 diabetes, with a focus on the balance of Th1- and Th2-like cytokines. Ten children with newly diagnosed type 1 diabetes (10-17 y) were treated with five double treatments of photopheresis and 10 children matched for disease, age, and gender were given placebo tablets and sham pheresis. Expression of IFN-γ and IL-4 mRNA was determined by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and secretion of IFN-γ, IL-10, and IL-13 in cell-culture supernatants by ELISA after stimulation with glutamic acid decarboxylase (GAD65) (a.a. 247-279), the ABBOS peptide (a.a. 152-169), insulin, phytohemagglutinin (PHA), and keyhole limpet hemocyanin (KLH). Photopheresis changed antigen-stimulated immune balance in line with a Th2-like shift. Thus, the ratio of IFN-γ/IL-4 mRNA expression after in vitro stimulation with a peptide of the autoantigen GAD 65 was reduced after treatment in the photopheresis group. The IFN-γ/IL-4 mRNA expression ratio after in vitro stimulation with insulin was also lower in children treated with photopheresis compared with the placebo group. Photopheresis has an immune-modulating effect in children with type 1 diabetes, causing a Th2-like deviation. Copyright © 2005 International Pediatric Research Foundation, Inc.

Place, publisher, year, edition, pages
2005. Vol. 58, no 3, 459-466 p.
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Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-29980DOI: 10.1203/01.pdr.0000176906.42001.c3Local ID: 15410OAI: oai:DiVA.org:liu-29980DiVA: diva2:250799
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2017-12-13

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Faresjö, MariaErnerudh, JanBerlin, GöstaLudvigsson, Johnny

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Faresjö, MariaErnerudh, JanBerlin, GöstaLudvigsson, Johnny
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Faculty of Health SciencesPediatricsClinical ImmunologyDepartment of Clinical Immunology and Transfusion MedicineTransfusion MedicineDepartment of Paediatrics in Linköping
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