Enhancement of anthraquinone accumulation in Morinda citrifolia suspension cultures
2005 (English)In: Plant Science, ISSN 0168-9452, Vol. 168, no 5, 1337-1344 p.Article in journal (Refereed) Published
Enhancement of accumulation of anthraquinones in Morinda citrifolia (Noni fruit) suspension cultures was accomplished by treatment with elicitors, by ultrasonication and by controlled feeding of the carbon source in the growth medium. The elicitation was attained by additions of polyunsaturated fatty acids (linoleic acid, α-linolenic acid, arachidonic acid), methyl jasmonate, salicylate and nitric oxide (by addition of sodium nitroprusside to the medium) at different concentrations. The accumulation of anthraquinones in the elicited cultures ranged from 5 to 12 mg/g dry weight of cells, which was two to three-fold of what was attained in control cultures. Treatment by short pulses of ultrasonication enhanced the accumulation up to 2.5-fold after 16 s of sonication. A synergistic effect was achieved by simultaneously applying elicitation and controlled addition of sucrose, that increased the anthraquinone production to 16.74 mg/g of dry weight, which was more than a four-fold increase above the control cultures. The accumulation of the anthraquinones in the M. citrifolia cells was confirmed by confocal laser fluorescence microscopy. Minor fluorescence was observed from the cells in the lag phase (1-3 days), while substantially higher fluorescence was observed at late exponential and stationary phase (10-14 days). Rounder spherical cells emitted less fluorescence than elongated slender cells. The fluorescence was assumed to be a result of autofluorescent properties of the aromatic anthraquinone molecules. © 2005 Elsevier Ireland Ltd. All rights reserved.
Place, publisher, year, edition, pages
2005. Vol. 168, no 5, 1337-1344 p.
Anthraquionone, suspension cultures, Morinda citrifolia
National CategoryNatural Sciences
IdentifiersURN: urn:nbn:se:liu:diva-30362DOI: 10.1016/j.plantsci.2005.01.017Local ID: 15909OAI: oai:DiVA.org:liu-30362DiVA: diva2:251184