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Transition state analysis of the complex between coagulation factor VIIa and tissue factor: Suggesting a sequential domain-binding pathway
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biochemistry.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biochemistry.
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2005 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 327, no 3, 789-793 p.Article in journal (Refereed) Published
Abstract [en]

Injury of a blood vessel exposes membrane-bound tissue factor (TF) to blood, which allows binding of coagulation factor VIIa (FVIIa). This initiation of the coagulation cascade is dictated by a specific multi-domain interaction between FVIIa and TF. To examine the energies involved in the transition state of the FVIIa:TF complex, various residues in the extracellular part of TF (sTF) that are known to interact with FVIIa were replaced with a smaller cysteine residue. Determination of Φ values in each of the positions using surface plasmon resonance measurements enabled us to characterize the transition state complex between the resulting sTF variants and FVIIa. We found that the interactions in the transition state seemed to be most pronounced between the protease domain of FVIIa and sTF while detailed specific interactions between the Gla-domain and sTF were missing. Thus, the transition state energy data indicate a sequential binding event between these two macromolecules. © 2004 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
2005. Vol. 327, no 3, 789-793 p.
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Natural Sciences
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URN: urn:nbn:se:liu:diva-30364DOI: 10.1016/j.bbrc.2004.12.058Local ID: 15911OAI: oai:DiVA.org:liu-30364DiVA: diva2:251186
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2017-12-13

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Österlund, MarieSvensson, MagdalenaCarlsson, Uno

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