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Sequential coagulation factor VIIa domain binding to tissue factor
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biochemistry.
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2005 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 337, no 4, 1276-1282 p.Article in journal (Refereed) Published
Abstract [en]

Vessel wall tissue factor (TF) is exposed to blood upon vascular damage which enables association with factor VIIa (FVIIa). This leads to initiation of the blood coagulation cascade through localization and allosteric induction of FVIIa procoagulant activity. To examine the docking pathway of the FVIIa-TF complex, various residues in the extracellular part of TF (sTF) that are known to interact with FVIIa were replaced with cysteines labelled with a fluorescent probe. By using stopped-flow fluorescence kinetic measurements in combination with surface plasmon resonance analysis, we studied the association of the resulting sTF variants with FVIIa. We found the docking trajectory to be a sequence of events in which the protease domain of FVIIa initiates contact with sTF. Thereafter, the two proteins are tethered via the first epidermal growth factor-like and finally the γ-carboxyglutamic acid (Gla) domain. The two labelled sTF residues interacting with the protease domain of FVIIa bind or become eventually ordered at different rates, revealing kinetic details pertinent to the allosteric activation of FVIIa by sTF. Moreover, when the Gla domain of FVIIa is removed the difference in the rate of association for the remaining domains is much more pronounced. © 2005 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
2005. Vol. 337, no 4, 1276-1282 p.
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Natural Sciences
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URN: urn:nbn:se:liu:diva-30378DOI: 10.1016/j.bbrc.2005.09.190Local ID: 15925OAI: oai:DiVA.org:liu-30378DiVA: diva2:251200
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2017-12-13

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Österlund, MarieCarlsson, UnoSvensson, Magdalena

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