The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)-L- homoserine lactone stimulates phagocytic activity in human macrophages through the p38 MAPK pathway
2005 (English)In: Microbes and infection, ISSN 1286-4579, Vol. 7, no 15, 1512-1518 p.Article in journal (Refereed) Published
Quorum-sensing is an important mechanism for the regulation of bacteria-to-bacteria communication. Recent advances have demonstrated that the Pseudomonas aeruginosa signaling molecule N-(3-oxododecanoyl)-L-homoserine lactone (3O-C12-HSL) is also a potent modulator of eukaryotic cells and may thus play an important role in the host response during P. aeruginosa infections. Little is known, however, about specific effects of 3O-C 12-HSL molecules on human macrophages. To address this issue, we investigated the influence of 3O-C12-HSL on the phagocytic activity, production of reactive oxygen species, and activation of p38 and p42/44 MAPK signaling pathways in human macrophages. We show an effect of 3O-C 12-HSL on the phagocytic capacity in human macrophages, which depends on concentration and time of exposure. When cells were exposed to 100 μM 3O-C12-HSL for 30 min or 1 h, the phagocytic activity increased 1.8 and 1.6 times, respectively. The 3O-C12-HSL treatments had no significant effect on the level of reactive oxygen species production. Furthermore, the p38 MAPK, but not the p42/44 MAPK, signaling pathway was activated in response to 3O-C12-HSL. In addition, specific blocking of p38 MAPK activation with 10 μM SB 203580 prevented the 3O-C 12-HSL-induced increase in the phagocytic activity. These findings demonstrate that the bacterial quorum-sensing can play a significant role also in regulation of macrophage activity during infections caused by P. aeruginosa. © 2005 Elsevier SAS. All rights reserved.
Place, publisher, year, edition, pages
2005. Vol. 7, no 15, 1512-1518 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-30846DOI: 10.1016/j.micinf.2005.05.012Local ID: 16502OAI: oai:DiVA.org:liu-30846DiVA: diva2:251669