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The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)-L- homoserine lactone stimulates phagocytic activity in human macrophages through the p38 MAPK pathway
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology.
Vilnius .
2005 (English)In: Microbes and infection, ISSN 1286-4579, E-ISSN 1769-714X, Vol. 7, no 15, 1512-1518 p.Article in journal (Refereed) Published
Abstract [en]

Quorum-sensing is an important mechanism for the regulation of bacteria-to-bacteria communication. Recent advances have demonstrated that the Pseudomonas aeruginosa signaling molecule N-(3-oxododecanoyl)-L-homoserine lactone (3O-C12-HSL) is also a potent modulator of eukaryotic cells and may thus play an important role in the host response during P. aeruginosa infections. Little is known, however, about specific effects of 3O-C 12-HSL molecules on human macrophages. To address this issue, we investigated the influence of 3O-C12-HSL on the phagocytic activity, production of reactive oxygen species, and activation of p38 and p42/44 MAPK signaling pathways in human macrophages. We show an effect of 3O-C 12-HSL on the phagocytic capacity in human macrophages, which depends on concentration and time of exposure. When cells were exposed to 100 μM 3O-C12-HSL for 30 min or 1 h, the phagocytic activity increased 1.8 and 1.6 times, respectively. The 3O-C12-HSL treatments had no significant effect on the level of reactive oxygen species production. Furthermore, the p38 MAPK, but not the p42/44 MAPK, signaling pathway was activated in response to 3O-C12-HSL. In addition, specific blocking of p38 MAPK activation with 10 μM SB 203580 prevented the 3O-C 12-HSL-induced increase in the phagocytic activity. These findings demonstrate that the bacterial quorum-sensing can play a significant role also in regulation of macrophage activity during infections caused by P. aeruginosa. © 2005 Elsevier SAS. All rights reserved.

Place, publisher, year, edition, pages
2005. Vol. 7, no 15, 1512-1518 p.
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Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-30846DOI: 10.1016/j.micinf.2005.05.012Local ID: 16502OAI: oai:DiVA.org:liu-30846DiVA: diva2:251669
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2017-12-13

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Vikström, OlenaMagnusson, Karl-Eric

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