liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Degradation of phenol under meso- and thermophilic, anaerobic conditions
Linköping University, The Tema Institute, Department of Water and Environmental Studies. Linköping University, Faculty of Arts and Sciences.
Linköping University, The Tema Institute, Department of Water and Environmental Studies. Linköping University, Faculty of Arts and Sciences.
Linköping University, The Tema Institute, Department of Water and Environmental Studies. Linköping University, Faculty of Arts and Sciences.
Linköping University, The Tema Institute, Department of Water and Environmental Studies. Linköping University, Faculty of Arts and Sciences.
1998 (English)In: Anaerobe, ISSN 1075-9964, E-ISSN 1095-8274, Vol. 5, no 1, 25-35 p.Article in journal (Refereed) Published
Abstract [en]

Based on the results of preliminary studies on phenol degradation under mesophilic conditions with a mixed methanogenic culture, we proposed a degradation pathway in which phenol is fermented to acetate: Part of the phenol is reductively transformed to benzoate while the rest is oxidised, forming acetate as end product. According to our calculations, this should result in three moles of phenol being converted to two moles of benzoate and three moles of acetate (3phenol+2CO2+3H2O→3acetate+2benzoate): To assess the validity of our hypothesis concerning the metabolic pathway, we studied the transformation of phenol under mesophilic and thermophilic conditions in relation to the availability of hydrogen. Hence, methanogenic meso- and thermophilic cultures amended with phenol were run with or without an added over-pressure of hydrogen under methanogenic and non-methanogenic conditions. Bromoethanesulfonic acid (BES) was used to inhibit methanogenic activity. In the mesophilic treatments amended with only BES, about 70% of the carbon in the products found was benzoate. During the course of phenol transformation in these BES-amended cultures, the formation pattern of the degradation products changed: Initially nearly 90% of the carbon from phenol degradation was recovered as benzoate, whereas later in the incubation, in addition to benzoate formation, the aromatic nucleus degraded completely to acetate. Thus, the initial reduction of phenol to benzoate resulted in a lowering of H2levels, giving rise to conditions allowing the degradation of phenol to acetate as the end product. Product formation in bottles amended with BES and phenol occurred in accordance with the hypothesised pathway; however, the overall results indicate that the degradation of phenol in this system is more complex.

During phenol transformation under thermophilic conditions, no benzoate was observed and no phenol was transformed in the BES-amended cultures. This suggests that the sensitivity of phenol transformation to an elevated partial pressure of H2is higher under thermophilic conditions than under mesophilic ones. The lack of benzoate formation could have been due to a high turnover of benzoate or to a difference in the phenol degradation pathway between the thermophilic and mesophilic cultures.

Place, publisher, year, edition, pages
1998. Vol. 5, no 1, 25-35 p.
Keyword [en]
phenol degradation, electronacceptor, acetate formation, meso and thermophilic conditions, methane formation
National Category
Social Sciences Interdisciplinary
Identifiers
URN: urn:nbn:se:liu:diva-31040DOI: 10.1006/anae.1998.0187Local ID: 16748OAI: oai:DiVA.org:liu-31040DiVA: diva2:251863
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2012-06-29Bibliographically approved
In thesis
1. Anaerobic degradation of phenol and related aromatics
Open this publication in new window or tab >>Anaerobic degradation of phenol and related aromatics
2000 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Phenol and other simple aromatic compounds have been found in water leached from landfills, showing that these types of compounds could be either present in disposed waste, or released from it via transformation and degradation processes. Hence, the fate anddegradation potential of such compounds under landfilling conditions is of great concern. Therefore, using micro-organisms from landfills, I have investigated the anaerobic biodegradation of phenol, dimethyl phthalate, 3-chlorobenzoate, 2,4,6-trichlorophenol, tetrabromophthalic acid and aniline. The compounds were chosen to represent substrates of potentially important reactions in the transformation and degradation of aromatic compounds. 24 waste samples from landfills and a time series of samples taken over five years from fourlandfill simulation reactors (in all 20 waste samples) were used as sources of microorganisms. The capacity of these waste samples to degrade the halogenated aromatics was poor or completely absent, indicating that halogenated compounds could be more persistent inlandfills than in other previously investigated anaerobic environments. Phenol and dimethyl phthalate were more readily transformed by most landfill samples, but the degradation capacity was poorer in the landfill simulation experiments. Here the unique sampling series showed an increase in degradation capacity with time, indicating that one to two years is needed to allow a micro-flora capable of degrading aromatic compounds to develop. However, the landfill samples showed higher degradation potentials than the simulation reactor samples, even from the later stages.

A more extensive study designed to elucidate the phenol degradation pathway under anaerobic, fermenting conditions is also presented. Here, phenol was for the first time shown to be degraded to non-aromatic products in a non-methanogenic fermenting culture. The endproductsformed were benzoate, acetate and butyrate. The conversion of phenol to benzoate was proved to be an electron sink reaction, used during processes such as degradation of glucose and is a new example of the diversity of compounds that can used as electronsinks/ acceptors in anaerobic environments. The degradation pathway in the studied cultureproceeds via fom1ation of 4-hydroxybenzoate, 4-hydroxybenzoyl-CoA and benzoyl-CoA, and the activity of a CoA-transferase which activates 4-hydroxybenzoate was measured.

Place, publisher, year, edition, pages
Motala: Kanaltryckeriet, 2000. 61 p.
Series
Linköping Studies in Arts and Science, ISSN 0282-9800 ; 212
Keyword
Aromatic compunds, anaerobic degradation, Fysik, Kemi
National Category
Social Sciences Interdisciplinary
Identifiers
urn:nbn:se:liu:diva-32129 (URN)17988 (Local ID)91-7219-728-5 (ISBN)17988 (Archive number)17988 (OAI)
Public defence
2000-05-31, Sal Elysion, Hus-T, Universitetsområdet Valla, Linköping, 10:15 (English)
Opponent
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2014-08-27Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Karlsson, AnnaEjlertsson, JörgenNezirevic, DzenetaSvensson, Bo H.

Search in DiVA

By author/editor
Karlsson, AnnaEjlertsson, JörgenNezirevic, DzenetaSvensson, Bo H.
By organisation
Department of Water and Environmental StudiesFaculty of Arts and Sciences
In the same journal
Anaerobe
Social Sciences Interdisciplinary

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 1400 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf