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CO2-dependent fermentation of phenol to acetate, butyrate and benzoate by an anaerobic, pasteurised culture
Linköping University, The Tema Institute, Department of Water and Environmental Studies. Linköping University, Faculty of Arts and Sciences.
Linköping University, The Tema Institute, Department of Water and Environmental Studies. Linköping University, Faculty of Arts and Sciences.
Linköping University, The Tema Institute, Department of Water and Environmental Studies. Linköping University, Faculty of Arts and Sciences.
2000 (English)In: Archives of Microbiology, ISSN 0302-8933, E-ISSN 1432-072X, Vol. 173, no 5-6, 398-402 p.Article in journal (Refereed) Published
Abstract [en]

Fermentative degradation of phenol was studied using a non-methanogenic, pasteurised enrichment culture containing two morphologically different bacteria. Phenol was fermented to benzoate, acetate and butyrate and their relative occurrence depended on the concentration of hydrogen. Proportionately more benzoate was formed with high initial levels of H2. The influence of P(H2) on the fermentation pattern was studied both in dense cell suspensions and in growing cultures by addition of hydrogen. An increase in growth yield (OD578 was observed, compared to controls, as a consequence of phenol degradation, however, the increase was less in H2-amended treatments, in which most of the phenol ended up as benzoate. The degradation of phenol in the dense cell suspension experiments was dependent on CO2. Benzoate was not degraded when added as a substrate to the growing culture. This is, to our knowledge, the first report concerning the fermentative degradation of phenol to nonaromatic products.

Place, publisher, year, edition, pages
2000. Vol. 173, no 5-6, 398-402 p.
Keyword [en]
Phenol fermentation, Reductive dehydroxylation, Hydrogen partial pressure, Ring cleavage
National Category
Social Sciences Interdisciplinary
Identifiers
URN: urn:nbn:se:liu:diva-32080DOI: 10.1007/s002030000160Local ID: 17935OAI: oai:DiVA.org:liu-32080DiVA: diva2:252902
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2012-06-29Bibliographically approved
In thesis
1. Anaerobic degradation of phenol and related aromatics
Open this publication in new window or tab >>Anaerobic degradation of phenol and related aromatics
2000 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Phenol and other simple aromatic compounds have been found in water leached from landfills, showing that these types of compounds could be either present in disposed waste, or released from it via transformation and degradation processes. Hence, the fate anddegradation potential of such compounds under landfilling conditions is of great concern. Therefore, using micro-organisms from landfills, I have investigated the anaerobic biodegradation of phenol, dimethyl phthalate, 3-chlorobenzoate, 2,4,6-trichlorophenol, tetrabromophthalic acid and aniline. The compounds were chosen to represent substrates of potentially important reactions in the transformation and degradation of aromatic compounds. 24 waste samples from landfills and a time series of samples taken over five years from fourlandfill simulation reactors (in all 20 waste samples) were used as sources of microorganisms. The capacity of these waste samples to degrade the halogenated aromatics was poor or completely absent, indicating that halogenated compounds could be more persistent inlandfills than in other previously investigated anaerobic environments. Phenol and dimethyl phthalate were more readily transformed by most landfill samples, but the degradation capacity was poorer in the landfill simulation experiments. Here the unique sampling series showed an increase in degradation capacity with time, indicating that one to two years is needed to allow a micro-flora capable of degrading aromatic compounds to develop. However, the landfill samples showed higher degradation potentials than the simulation reactor samples, even from the later stages.

A more extensive study designed to elucidate the phenol degradation pathway under anaerobic, fermenting conditions is also presented. Here, phenol was for the first time shown to be degraded to non-aromatic products in a non-methanogenic fermenting culture. The endproductsformed were benzoate, acetate and butyrate. The conversion of phenol to benzoate was proved to be an electron sink reaction, used during processes such as degradation of glucose and is a new example of the diversity of compounds that can used as electronsinks/ acceptors in anaerobic environments. The degradation pathway in the studied cultureproceeds via fom1ation of 4-hydroxybenzoate, 4-hydroxybenzoyl-CoA and benzoyl-CoA, and the activity of a CoA-transferase which activates 4-hydroxybenzoate was measured.

Place, publisher, year, edition, pages
Motala: Kanaltryckeriet, 2000. 61 p.
Series
Linköping Studies in Arts and Science, ISSN 0282-9800 ; 212
Keyword
Aromatic compunds, anaerobic degradation, Fysik, Kemi
National Category
Social Sciences Interdisciplinary
Identifiers
urn:nbn:se:liu:diva-32129 (URN)17988 (Local ID)91-7219-728-5 (ISBN)17988 (Archive number)17988 (OAI)
Public defence
2000-05-31, Sal Elysion, Hus-T, Universitetsområdet Valla, Linköping, 10:15 (English)
Opponent
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2014-08-27Bibliographically approved

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Karlsson, AnnaSvensson, BoEjlertsson, Jörgen

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