liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
External quality assessment for molecular detection of Bordetella pertussis in European laboratories.
Show others and affiliations
2005 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 43, no 1, 30-35 p.Article in journal (Refereed) Published
Abstract [en]

Although the PCR for the detection of Bordetella pertussis is routinely performed in diagnostic laboratories, no quality assessment program has so far been described. We report on the results obtained with two external quality assessment proficiency panels sent to European laboratories. The first proficiency panel contained a series of dilutions of three previously characterized B. pertussis clinical isolates and two negative controls. No false-positive results were reported by six laboratories providing seven data sets. The reported limits of detection of the three B. pertussis strains varied between 4 and 4,000, 9 and 9,000, and 3 and 30,000 CFU/ml, respectively. The second proficiency panel, composed of a series of dilutions of reference strains of B. pertussis, B. holmesii, B. hinzii, and B. bronchiseptica, as well as negative controls, was sent to nine laboratories. One laboratory reported a negative result for a sample and reported a B. parapertussis-positive sample to be positive for B. pertussis. By using the B. pertussis-specific target gene pertactin, one laboratory detected B. pertussis with 100% specificity. All other laboratories, which used IS481-based assays, reported positive results for the samples containing B. holmesii and B. bronchiseptica, species that have occasionally been recovered from human respiratory samples. These data show that the choice of the target gene is particularly critical for the species specificity of B. pertussis PCR assays.

Place, publisher, year, edition, pages
2005. Vol. 43, no 1, 30-35 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-32351DOI: 10.1128/JCM.43.1.30-35.2005Local ID: 18246OAI: diva2:253173
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2010-05-20

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Olcén, Per
In the same journal
Journal of Clinical Microbiology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 33 hits
ReferencesLink to record
Permanent link

Direct link