liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
The por A pseudogene of Neisseria gonorrhoeae - low level of genetic polymorphism and a few, mainly identical, inactivating mutations.
Örebro University Hospital.
Örebro University Hospital.
Örebro University Hospital.
2005 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 113, no 6, 410-419 p.Article in journal (Refereed) Published
Abstract [en]

N. meningitidis is the only Neisseria species known to express two outer membrane porins, PorA and PorB. However, a porA pseudogene has been identified in N. gonorrhoeae. The present study investigated the prevalence and genetic polymorphism of this porA pseudogene in 87 different N. gonorrhoeae strains. The porA pseudogene was identified in all isolates. The pseudogene comprised 12 (5.5%), of which 10 were located in the promoter spacer, and 11 (1.0%) polymorphic nucleotide sites in the upstream segment containing the promoter region, i.e. the putative -10 and -35 sequences and the promoter spacer in-between, and the hypothetical PorA coding sequence, respectively. A phylogenetic analysis of the upstream segment and the hypothetical coding sequence identified 36 sequence variants, of which 30 were not previously described. All strains comprised at least two identical confirmed inactivating deletions, of which one was located in the promoter region and one in the hypothetical PorA coding sequence. In conclusion, the porA pseudogene and its few inactivating mutations are widespread in the N. gonorrhoeae population and the homology with the N. meningitidis porA gene reflects their common evolutionary origin. The highly conserved N. gonorrhoeae porA pseudogene may reflect an evolutionary neutral molecular clock and may be a suitable genetic target for diagnosis of N. gonorrhoeae.

Place, publisher, year, edition, pages
2005. Vol. 113, no 6, 410-419 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-32358DOI: 10.1111/j.1600-0463.2005.apm_206.xLocal ID: 18253OAI: diva2:253180
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2010-05-19

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Unemo, MagnusFredlund, Hans
In the same journal
Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS)
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 22 hits
ReferencesLink to record
Permanent link

Direct link