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Interlaboratory Comparison of PCR-Based Identification and Genogrouping of Neisseria meningitidis
Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France.
Neisseria Unit and the French National Reference Center for Meningococci, Institut Pasteur, Paris, France.
Irish Meningococcal and Meningitis Reference Laboratory, The Children's University Hospital, Dublin, Ireland.
WHO Collaborating Center for Reference and Research on Meningococci, Division of Infectious Disease Control, Norwegian Institute of Public Health and Institute of Oral Biology, University of Oslo, Oslo, Norway.
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2005 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 43, no 1, 144-149 p.Article in journal (Refereed) Published
Abstract [en]

Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.

Place, publisher, year, edition, pages
2005. Vol. 43, no 1, 144-149 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-33470DOI: 10.1128/JCM.43.1.144-149.2005Local ID: 19493OAI: diva2:254293
Available from: 2009-10-09 Created: 2009-10-09 Last updated: 2012-08-21Bibliographically approved

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Olcén, Per
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