Interlaboratory Comparison of PCR-Based Identification and Genogrouping of Neisseria meningitidis
2005 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 43, no 1, 144-149 p.Article in journal (Refereed) Published
Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.
Place, publisher, year, edition, pages
2005. Vol. 43, no 1, 144-149 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-33470DOI: 10.1128/JCM.43.1.144-149.2005Local ID: 19493OAI: oai:DiVA.org:liu-33470DiVA: diva2:254293