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Basophil allergen threshold sensitivity: A useful approach to anti-IgE treatment efficacy evaluation
Dept of Medicine KI, Stockholm.
Dept of Medicine KI, Stockholm.
Dept of Medicine, Lund.
Dept of Medicine Karolinska Hosp, Huddinge.
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2006 (English)In: Allergy. European Journal of Allergy and Clinical Immunology, ISSN 0105-4538, Vol. 61, no 3, 298-302 p.Article in journal (Refereed) Published
Abstract [en]

Background: Monitoring of the allergen sensitivity of a patient is most important for optimal patient care and a basic prerequisite for immunomodulating treatment. The objective of this study was to investigate how basophil allergen sensitivity can be applied in the monitoring of anti-immunoglobulin E (IgE) treatment. Methods: Basophils from timothy grass pollen allergic patients were, by flow cytometry, analysed for allergen threshold sensitivity (CD-sens) by measuring CD63 up-regulation on CD203c-identified basophils. The results were compared with maximal percentage CD63 up-regulation at one allergen dose (CD-max), skin prick test end-point allergen titration, (SPT-sens), nasal provocation titration tests (nasal provocation titre) and serum IgE and IgE antibody concentrations. Results: There was a significant correlation (r = 0.50, P = 0.01) between CD-sens and SPT-sens, CD-sens and the IgE antibody concentration in percentage of 'total IgE' (relative IgE antibody concentration) (r = 0.72, P < 0.001) as well as between CD-sens and nasal provocation titre (r = 0.54, P < 0.05) but, in contrast, CD-max did not correlate with any of the sensitization parameters, i.e. SPT-sens, nasal provocation titre, absolute and relative IgE antibody concentration or CD-sens. CD-sens could be used to monitor omalizumab treatment efficacy while, based on CD-max, four of seven symptom-free patients on omalizumab would have been classified as having ongoing allergy. Conclusions: CD-sens seems to be very useful for the determination of a patient's allergen sensitivity and should be evaluated for the measurement and monitoring of anti-IgE treatment efficacy. CD-max, the conventional approach to basophil allergen challenge, which mirrors cell reactivity, gives incorrect information. Copyright © Blackwell Munksgaard 2006.

Place, publisher, year, edition, pages
2006. Vol. 61, no 3, 298-302 p.
Keyword [en]
CD-sens, CD203c, CD63, immunoglobulin E, omalizumab
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-34821DOI: 10.1111/j.1398-9995.2006.00987.xLocal ID: 23433OAI: diva2:255669
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-11

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Irander, Kristina
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