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Importin beta: A novel autoantigen in human autoimmunity identified by screening random peptide libraries on phage
Queen Mary College, London, UK.
Queen Mary College, London, UK.
Queen Mary College, London, UK.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Pediatrics. Östergötlands Läns Landsting, Centre of Paediatrics and Gynecology and Obstetrics, Department of Paediatrics in Linköping.
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2006 (English)In: Journal of Autoimmunity, ISSN 0896-8411, Vol. 26, no 3, 197-207 p.Article in journal (Refereed) Published
Abstract [en]

By screening random peptide libraries (RPLs) with sera of Type 1 diabetes (T1D) patients, we previously identified 5 disease-specific 'mimotopes' displayed on phages (phagotopes). We already characterised 1 phagotope (CH1p), as an epitope of human osteopontin, an autoantigen expressed within the somatostatin cells of human islets. In this paper, we report the characterization of the second phagotope, 195Dyn, by immunohistochemistry, Western Blotting and screening of a human islet cDNA library using rabbit anti-195Dyn antibodies. The 195Dyn mimotope was detected in human islets. The screening of a λgt11 cDNA library from human islets has identified a clone, which corresponded to human importin beta. ELISA detected autoantibodies against this protein in sera of around 60% of TD1 patients and in 30% of patients affected by other autoimmune diseases. In summary, RPLs technology proved again successful in identifying another novel autoantigen (importin beta), whose significance in the autoimmune process remains to be fully elucidated. © 2006 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
2006. Vol. 26, no 3, 197-207 p.
Keyword [en]
autoantigens, autoimmunity, peptide libraries
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-34824DOI: 10.1016/j.jaut.2006.01.003Local ID: 23451OAI: diva2:255672
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-11

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Ludvigsson, Johnny
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Faculty of Health SciencesPediatricsDepartment of Paediatrics in Linköping
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