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Pooling ambulatory saliva cortisol samples over consecutive days – as reliable as arithmetic means
Linköping University, Department of Department of Health and Society, Division of Preventive and Social Medicine and Public Health Science. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Medical and Health Sciences, Health and Society. Linköping University, Faculty of Arts and Sciences.
Linköping University, Department of Department of Health and Society, Division of Preventive and Social Medicine and Public Health Science. Linköping University, Faculty of Health Sciences. (Landstinget i Östergötland; Centre for Public Health Sciences; Centre for Public Health Sciences; Folkhälsovetenskapligt centrum; Folkhälsovetenskapligt centrum)
2008 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 68, no 6, 508-512 p.Article in journal (Refereed) Published
Abstract [en]

Objective: When cortisol measurements are to be studied in large populations, cost-effective analyses are needed. This study aimed at testing whether one pooled cortisol value over three consecutive days is as reliable as using the arithmetic mean of the samples from the same measure points.

Material and methods: Thirty participants aged between 45 and 69 collected saliva in salivettes immediately after awakening (t1), 30 min after awakening (t2) and in the evening (t3) during 3 consecutive days. A fixed volume from each of the samples (t1, t2 and t3) was pooled prior to laboratory analysis. Mean levels over 3 days for t1, t2 and t3 were compared to corresponding levels of pooled vials. Cortisol levels were analysed using a radio immunoassay.

Results: All measures tested had high correlations between mean values and pooled samples, exemplified with diurnal deviation rdif t2–t350.974 (CI 0.946;0.987), and awakening response rdif t2–t150.982 (CI 0.963;0.991). There were no statistical differences between the pooled values and the arithmetic means.

Conclusion: Pooling samples gave as reliable results as arithmetic means did. Pooling samples prior to laboratory analysis is a cost-effective method for measuring general diurnal cortisol variation in field research projects.

Place, publisher, year, edition, pages
2008. Vol. 68, no 6, 508-512 p.
Keyword [en]
Cortisol, HPA-axis, measurements, methodology, saliva, stress
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-14927DOI: 10.1080/00365510701832229OAI: oai:DiVA.org:liu-14927DiVA: diva2:25615
Note

Original publication: Peter Garvin, John Carstensen and Margareta Kristenson, Pooling ambulatory saliva cortisol samples over consecutive days – as reliable as arithmetic means, 2008, Scandinavian Journal of Clinical and Laboratory Investigation, (68), 6, 508-512. http://dx.doi.org/10.1080/00365510701832229. Copyright © Taylor & Francis Group, an informa business

Available from: 2008-09-30 Created: 2008-09-30 Last updated: 2017-12-13
In thesis
1. Plasma levels of matrix metalloproteinase‐9 in a normal population: a psychoneuroendocrinological approach
Open this publication in new window or tab >>Plasma levels of matrix metalloproteinase‐9 in a normal population: a psychoneuroendocrinological approach
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Several large‐scale epidemiological studies have demonstrated the prognostic significance of psychosocial factors and stress for coronary artery disease (CAD). Observations of sudden changes in CAD incidence have led to the proposal of mechanisms regarding atherosclerotic plaque vulnerability. The collagen‐degrading enzyme matrix metalloproteinase-9 (MMP-9) is increased in rupture‐prone plaques with high inflammatory activity, and circulating levels of MMP-9 are raised in patients with acute coronary syndrome. However, the distribution of MMP‐9 levels and its relations to psychosocial factors and the stress hormone cortisol have not been previously explored in a normal population.The aim of this dissertation was to examine in a normal population the association of circulating levels of MMP-9 with traditional cardiovascular risk factors including levels of C-reactive protein (CRP), with psychosocial factors, and with saliva levels of cortisol. In addition, the reliability of a new method of ambulatory saliva sampling for assessment of cortisol levels was evaluated. A sub‐sample of the Life conditions, Stress, and Health (LSH)-study, a population based study exploring psychoneuroendocrinological pathways mediating the differences in CAD incidence over socioeconomic status, was used. Plasma levels of MMP-9 were examined in a sample randomly drawn from the LSH‐study (n=400), aged 45 to 69 years at enrollment.The main findings were: 1) there was a positive association between plasma MMP-9 levels and total risk load of cardiovascular risk factors. The findings were persistent after adjusting for CRP and could not be attributed to a single risk factor. 2) After adjusting for traditional cardiovascular risk factors and CRP, MMP-9 levels were positively associated with psychosocial risk factors and negatively associated with psychosocial resources. 3) Pooling saliva samples prior to laboratory analysis were as reliable as arithmetic means for assessment of diurnal cortisol variation in a field research setting. 4) There was a positive association between circulating levels of MMP‐9 and saliva levels of cortisol, both diurnal peak level and evening level of cortisol. The observed associations between MMP‐9 and traditional cardiovascular risk factors, psychosocial factors, and saliva cortisol levels suggest a psychoneuroendocrinological pathway linking stress to plaque vulnerability and provide increased understanding of the association between psychosocial factors and CAD.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2008. 131 p.
Series
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1072
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:liu:diva-14929 (URN)978‐91‐7393‐831‐0 (ISBN)
Public defence
2008-10-03, Berzeliussalen, ingång 63, Campus US, Linköpings universitet, Linköping, 13:00 (English)
Opponent
Supervisors
Available from: 2008-09-30 Created: 2008-09-30 Last updated: 2009-08-21Bibliographically approved

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Garvin, PeterCarstensen, JohnKristenson, Margareta

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