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Proteome profiling of corneal epithelium and identification of marker proteins for keratoconus, a pilot study
Linköping University, Faculty of Health Sciences. Linköping University, Department of Neuroscience and Locomotion, Ophthalmology. Östergötlands Läns Landsting, Reconstruction Centre, Department of Ophthalmology UHL.
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2006 (English)In: Experimental Eye Research, ISSN 0014-4835, Vol. 82, no 2, 201-209 p.Article in journal (Refereed) Published
Abstract [en]

The purpose of this study is to identify corneal proteins differentially expressed between keratoconus and normal epithelial samples. Proteins from the corneal epithelium were isolated from 6 keratoconus and 6 myopia patients (controls) and separated by 2D-gel electrophoresis. Six % and 12% SDS-PAGE gels were used to separate low and high molecular weight proteins. Gels were silver stained and protein spots were defined by Melanie II software. The proteins that were most altered in expression comparing keratoconus and controls were extracted, trypsin-digested, and identified by mass spectroscopy. Approximately 200-500 protein spots were detected on each gel. Nineteen spots were identified as differentially expressed between keratoconus and reference epithelium including cytokeratin 3 (<7.8 fold), gelsolin (1.6 fold), S100A4 (1.9 fold), and enolase 1 (0.72 fold). Another identified protein found at very high levels was cytokeratin 12. Gelsolin, cytokeratin 3, and cytokeratin 12 have previously been described to be involved in other corneal diseases. Three proteins, gelsolin, alpha enolase, and S100A4 were identified to be differentially expressed in keratoconus compared to reference epithelium and thus may be involved in the pathogenesis. © 2005 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
2006. Vol. 82, no 2, 201-209 p.
Keyword [en]
cornea; keratoconus; 2D-gel; SDS-PAGE; silver staining; proteomics
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-35657DOI: 10.1016/j.exer.2005.06.009Local ID: 28059OAI: diva2:256505
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-11

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Fagerholm, Per
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Faculty of Health SciencesOphthalmologyDepartment of Ophthalmology UHL
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