Progastrin-releasing peptide and gastrin-releasing peptide receptor mRNA expression in non-tumor tissues of the human gastrointestinal tract
2006 (English)In: World Journal of Gastroenterology, ISSN 1007-9327, Vol. 12, no 16, 2574-2578 p.Article in journal (Refereed) Published
Aim: To investigate the expression of gastrin-releasing peptide (GRP) and GRP-receptor mRNA in non-tumor tissues of the human esophagus, gastrointestinal tract, pancreas and gallbladder using molecular biology techniques. Methods: Poly A+ mRNA was isolated from total RNA extracts using an automated nucleic acid extractor and, subsequently, converted into single-stranded cDNA (ss-cDNA). PCR amplifications were carried out using gene-specific GRP and GRP-receptor primers. The specificity of the PCR amplicons was further confirmed by Southern blot analyses using gene-specific GRP and GRP-receptor hybridization probes. Results: Expre ssion of GRP and GRP-receptor mRNA was detected at various levels in nearly all segments of the non-tumor specimens analysed, except the gallbladder. In most of the biopsy specimens, co-expression of both GRP and GRP-receptor mRNA appeared to take place. However, expression of GRP mRNA was more prominent than was GRP-receptor mRNA. Conclusion: GRP and GRP-receptor mRNAs are expressed throughout the gastrointestinal tract and provides information for the future mapping and determination of its physiological importance in normal and tumor cells. © 2006 The WJG Press. All rights reserved.
Place, publisher, year, edition, pages
2006. Vol. 12, no 16, 2574-2578 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-35820Local ID: 28649OAI: oai:DiVA.org:liu-35820DiVA: diva2:256668