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Characterization of SCP-2 from Euphorbia lagascae reveals that a single Leu/Met exchange enhances sterol transfer activity
Department of Biochemistry and Pharmacy ° bo Akademi University, Turku, Finland.
Department of Biochemistry and Pharmacy ° bo Akademi University, Turku, Finland.
Department of Plant Biology and Forest Genetics Swedish University of Agricultural Sciences, Uppsala, Sweden.
Department of Plant Biology and Forest Genetics Swedish University of Agricultural Sciences, Uppsala, Sweden.
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2006 (English)In: The FEBS Journal, ISSN 1742-464X, E-ISSN 1742-4658, Vol. 273, 5641-5655 p.Article in journal (Refereed) Published
Abstract [en]

Sterol carrier protein-2 (SCP-2) is a small intracellular basic protein domain implicated in peroxisomal beta-oxidation. We extend our knowledge of plant SCP-2 by characterizing SCP-2 from Euphorbia lagascae. This protein consists of 122 amino acids including a PTS1 peroxisomal targeting signal. It has a molecular mass of 13.6 kDa and a pI of 9.5. It shares 67% identity and 84% similarity with SCP-2 from Arabidopsis thaliana. Proteomic analysis revealed that E. lagascae SCP-2 accumulates in the endosperm during seed germination. It showed in vitro transfer activity of BODIPY-phosphatidylcholine (BODIPY-PC). The transfer of BODIPY-PC was almost completely inhibited after addition of phosphatidylinositol, palmitic acid, stearoyl-CoA and vernolic acid, whereas sterols only had a very marginal inhibitory effect. We used protein modelling and site-directed mutagenesis to investigate why the BODIPY-PC transfer mediated by E. lagascae SCP-2 is not sensitive to sterols, whereas the transfer mediated by A. thaliana SCP-2 shows sterol sensitivity. Protein modelling suggested that the ligand-binding cavity of A. thaliana SCP-2 has four methionines (Met12, 14, 15 and 100), which are replaced by leucines (Leu11, 13, 14 and 99) in E. lagascae SCP-2. Changing Leu99 to Met99 was sufficient to convert E. lagascae SCP-2 into a sterol-sensitive BODIPY-PC-transfer protein, and correspondingly, changing Met100 to Leu100 abolished the sterol sensitivity of A. thaliana SCP-2.

Place, publisher, year, edition, pages
2006. Vol. 273, 5641-5655 p.
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Natural Sciences
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URN: urn:nbn:se:liu:diva-36509DOI: 10.1111/j.1742-4658.2006.05553.xLocal ID: 31470OAI: oai:DiVA.org:liu-36509DiVA: diva2:257357
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13

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Edqvist, Johan

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