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Bone marrow dendritic cells internalize live RF-81 bovine rotavirus and rotavirus-like particles (RF 2/6-GFP-VLP and RF 8*2/6/7-VLP) but are only activated by live bovine rotavirus
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2007 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, Vol. 65, no 6, 494-502 p.Article in journal (Refereed) Published
Abstract [en]

Dendritic cells (DC) represent the link between innate and adaptive immunity. They are classified as antigen-presenting cells (APC) and can initiate and modulate the immune response. To investigate the interaction with DCs, live RF-81 bovine rotavirus strain (RFV) and rotavirus-like particles (rota-VLP), RF 2/6-GFP-VLP and rota RF 8*2/6/7-VLP, were added in vitro to murine bone marrow-derived DCs (bmDCs). Live RFV, RF 2/6-GFP-VLP and RF 8*2/6/7-VLP all bound to bmDC and were internalized but only live RFV stimulated phenotypic maturation of the bmDCs as shown by the upregulation of the co-stimulatory molecule CD86. Even though bmDCs internalized RF 2/6-GFP-VLP and RF 8*2/6/7-VLP as efficiently as live RFV, these rota-VLP were not able to activate the cells. Supernatants derived from bmDC cultures treated with live RFV, RF 2/6-GFP-VLP or RF 8*2/6/7-VLP were examined for TNF-α production. At 6, 18 and 24 h post-infection, TNF-α concentrations were significantly increased in cultures treated with live RFV and rota-VLP compared with untreated cultures. In conclusion, this study showed that live RF-81 bovine rotavirus strain was internalized and induced bmDCs activation, whereas both RF 2/6-GFP-VLP and RF 8*2/6/7-VLP were internalized by bmDCs without triggering their activation. © 2007 The Authors.

Place, publisher, year, edition, pages
2007. Vol. 65, no 6, 494-502 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-38146DOI: 10.1111/j.1365-3083.2007.01907.xLocal ID: 41966OAI: diva2:258995
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-11

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Larsson, MarieMagnusson, Karl-EricSvensson, LennartHinkula, Jorma
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Faculty of Health SciencesMolecular VirologyMedical Microbiology
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