liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Basolateral entry and release of Crimean-Congo hemorrhagic fever virus in polarized MDCK-1 cells
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology.
2007 (English)In: Journal of Virology, ISSN 0022-538X, Vol. 81, no 5, 2158-2164 p.Article in journal (Refereed) Published
Abstract [en]

Crimean-Congo hemorrhagic fever virus (CCHFV) is an etiological agent of a disease with mortality rates in patients averaging 30%. The disease is characterized by fever, myalgia, and hemorrhage. Mechanisms underlying the hemorrhage have to our knowledge not been elucidated for CCHFV. Possibly, a direct or indirect viral effect on tight junctions (TJ) could cause the hemorrhage observed in patients, as TJ play a crucial role in vascular homeostasis and can cause leakage upon deregulation. Moreover, there is no knowledge regarding the site of entry and release of CCHFV in polarized epithelial cells. Such cells represent a barrier to virus dissemination within the host, and as a site of viral entry and release, they could play a key role in further spread. For the first time, we have shown preferential basolateral entry of CCHFV in Madin-Darby canine kidney 1 (MDCK-1) epithelial cells. Furthermore, we demonstrated basolateral release of CCHFV in polarized epithelial cells. Interestingly, by measuring transepithelial electrical resistance, we found no effect of CCHFV replication on the function of TJ in this study. Neither did we observe any difference in the localization of the TJ proteins ZO-1 and occludin in CCHFV-infected cells compared to mock-infected cells. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Place, publisher, year, edition, pages
2007. Vol. 81, no 5, 2158-2164 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-38152DOI: 10.1128/JVI.02070-06Local ID: 42089OAI: diva2:259001
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-11

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Magnusson, Karl-Eric
By organisation
Faculty of Health SciencesMedical Microbiology
In the same journal
Journal of Virology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 15 hits
ReferencesLink to record
Permanent link

Direct link