Pseudomonas aeruginosa quorum sensing molecule N-(3 oxododecanoyl)-l-homoserine lactone disrupts epithelial barrier integrity of Caco-2 cells
2006 (English)In: FEBS Letters, ISSN 0014-5793, Vol. 580, no 30, 6921-6928 p.Article in journal (Refereed) Published
Acyl-homoserine lactone (HSL) quorum sensing molecules play an important role in regulation of virulence gene expression in Pseudomonas aeruginosa. Here, we show that 3O-C12-HSL can disrupt barrier integrity in human epithelial Caco-2 cells as evidenced by decreased transepithelial electrical resistance (TER), increased paracellular flux, reduction in the expression and distribution of ZO-1 and occludin, and reorganization of F-actin. P. aeruginosa 3O-C12-HSL activate p38 and p42/44 kinases, and inhibition of these kinases partly prevented 3O-C12-HSL-induced changes in TER, paracellular flux and expression of occludin and ZO-1. These findings demonstrate that P. aeruginosa 3O-C12-HSL can modulate tight junction integrity of Caco-2 cells. © 2006 Federation of European Biochemical Societies.
Place, publisher, year, edition, pages
2006. Vol. 580, no 30, 6921-6928 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-38153DOI: 10.1016/j.febslet.2006.11.057Local ID: 42090OAI: oai:DiVA.org:liu-38153DiVA: diva2:259002