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Surface plasmon resonance detection of blood coagulation and platelet adhesion under venous and arterial shear conditions
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Rheumatology.ORCID iD: 0000-0002-6916-5490
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Sensor Science and Molecular Physics.
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2007 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 23, no 2, 261-268 p.Article in journal (Refereed) Published
Abstract [en]

A surface plasmon resonance (SPR) based flow chamber device was designed for real time detection of blood coagulation and platelet adhesion in platelet rich plasma (PRP) and whole blood. The system allowed the detection of surface interactions throughout the 6 mm length of the flow chamber. After deposition of thromboplastin onto a section of the sensor surface near the inlet of the flow chamber, coagulation was detected downstream of this position corresponding to a SPR signal of 7 to 8 mRIU (7 to 8 ng/mm2). A nonmodified control surface induced coagulation 3.5 times slower. Platelet adhesion to gold and fibrinogen coated surfaces in the magnitude of 1.25 and 1.66 mRIU was also shown with platelets in buffer, respectively. SPR responses obtained with PRP and whole blood on surfaces that were methylated or coated with von Willebrand factor (vWF), fibrinogen, or collagen, coincided well with platelet adhesion as observed with fluorescence microscopy in parallel experiments. The present SPR detection equipped flow chamber system is a promising tool for studies on coagulation events and blood cell adhesion under physiological flow conditions, and allows monitoring of short-range surface processes in whole blood. © 2007 Elsevier B.V. All rights reserved.

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2007. Vol. 23, no 2, 261-268 p.
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Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-39210DOI: 10.1016/j.bios.2007.04.009Local ID: 47285OAI: oai:DiVA.org:liu-39210DiVA: diva2:260059
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13

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Hansson, KennyJohansen, KnutWetterö, JonasKlenkar, GoranBenesch, JohanLundström, IngemarLindahl, TomasTengvall, Pentti

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Hansson, KennyJohansen, KnutWetterö, JonasKlenkar, GoranBenesch, JohanLundström, IngemarLindahl, TomasTengvall, Pentti
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The Institute of TechnologyDepartment of Physics, Chemistry and BiologyFaculty of Health SciencesRheumatologySensor Science and Molecular PhysicsApplied PhysicsDivision of clinical chemistryDepartment of Clinical Chemistry
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