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Expression of the focal adhesion protein PINCH in normal and alkali-injured corneas and the role of PMNs
Linköping University, Faculty of Health Sciences. Linköping University, Department of Neuroscience and Locomotion, Ophthalmology. Östergötlands Läns Landsting, Reconstruction Centre, Department of Ophthalmology UHL.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Neuroscience and Locomotion, Ophthalmology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Biomedicine and Surgery, Oncology. Östergötlands Läns Landsting, Centre of Surgery and Oncology, Department of Oncology UHL.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Biomedicine and Surgery, Oncology. Östergötlands Läns Landsting, Centre of Surgery and Oncology, Department of Oncology UHL.
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2007 (English)In: Acta Ophthalmologica Scandinavica, ISSN 1395-3907, E-ISSN 1600-0420, Vol. 85, no 4, 395-400 p.Article in journal (Refereed) Published
Abstract [en]

Purpose: To evaluate the role of particularly interesting new cysteine-histidine-rich protein (PINCH) in corneal wound healing and early neovascularization and to assess the influence of granulocytes. Methods: A standardized corneal alkali wound was inflicted under general anaesthesia to the right eye of 14 New Zealand White rabbits. Seven of the rabbits received i.v. 5 mg/kg fucoidin every 2 hours to prevent granulocytes from entering the wound area. After 36 hours, the rabbits were killed, the corneas excised, fixed in 4% formaldehyde and embedded in paraffin. The sections were double-stained with antibodies against PINCH and with haematoxylin. Results: In the normal cornea and limbus, PINCH was weakly expressed in the corneal epithelium and in a wedge of the conjunctival stroma. In the wounded corneas, PINCH expression was seen in the frontline of repopulating endothelial and epithelial cells, and in active keratocytes. The vascular endothelium and the granulocytes expressed PINCH, as did the conjunctival epithelium. In the fucoidin-treated rabbits, PINCH expression was markedly reduced. The vascular endothelial cells and the few granulocytes did not express PINCH in these rabbits. Conclusions: PINCH is only slightly expressed in the normal cornea. A corneal wound induces PINCH expression in the repopulating cells, in the vascular endothelial cells of the limbus, in the limbal epithelium and in the granulocytes. Exclusion of granulocytes reduces expression of PINCH and there is no expression at all in the vascular endothelium. © 2007 The Authors Journal compilation 2007 Acta Ophthalmol Scand.

Place, publisher, year, edition, pages
2007. Vol. 85, no 4, 395-400 p.
Keyword [en]
cornea - rabbit - wound healing - PINCH - neovascularization
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-39238DOI: 10.1111/j.1600-0420.2006.00861.xLocal ID: 47594OAI: oai:DiVA.org:liu-39238DiVA: diva2:260087
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13

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Bourghardt Peebo, BeatriceGan, LishaSun, Xiao-FengKnutsen Holmqvist, AnnicaFagerholm, Per

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Bourghardt Peebo, BeatriceGan, LishaSun, Xiao-FengKnutsen Holmqvist, AnnicaFagerholm, Per
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Faculty of Health SciencesOphthalmologyDepartment of Ophthalmology UHLOncologyDepartment of Oncology UHL
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Acta Ophthalmologica Scandinavica
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