Radiotherapy response in oral squamous carcinoma cell lines: Evaluation of apoptotic proteins as prognostic factors
2007 (English)In: Head and Neck, ISSN 1043-3074, E-ISSN 1097-0347, Vol. 29, no 4, 325-334 p.Article in journal (Refereed) Published
Background. In this study, we investigated the importance of apoptosis for cell death after radiotherapy, and whether the expression of pro- and anti-apoptotic proteins has any correlation to the radiosensitivity.
Methods. Three oral squamous cell carcinoma cell lines, UT-SCC-2, UT-SCC-9 and UT-SCC-24A, were subjected to radiotherapy. After irradiation, viable and dead cells were counted to determine radiation sensitivity and apoptosis was analyzed by measurement of caspase-3 activity. The expressions of pro- and anti-apoptotic proteins were assessed using western blot analyses.
Results and Conclusion. After irradiation, apoptotic morphology and caspase-3 activity were only detected in cell lines exhibiting high or moderate radiosensitivity. Western blot analysis indicates that survivin, epidermal growth factor receptor, cyclooxygenase-2, and Bcl-xL are critical components in irradiation resistance of the investigated cell lines. Moreover, our results suggest that apoptotic cell death and the balance between pro- and anti-apoptotic proteins are of importance for the outcome of radiotherapy.
Place, publisher, year, edition, pages
2007. Vol. 29, no 4, 325-334 p.
Apoptosis; oral tumors; predictive markers; radiotherapy; radiosensitivity
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-39259DOI: 10.1002/hed.20520Local ID: 47684OAI: oai:DiVA.org:liu-39259DiVA: diva2:260108