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Standardisation of operating procedures for the detection of minimal disease by QRT-PCR in children with neuroblastoma: Quality assurance on behalf of SIOPEN-R-NET
Linköping University, Faculty of Health Sciences. Linköping University, Department of Biomedicine and Surgery, Division of clinical chemistry. Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Chemistry.
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2007 (English)In: European Journal of Cancer, ISSN 0959-8049, Vol. 43, no 2, 341-350 p.Article in journal (Refereed) Published
Abstract [en]

The clinical utility of detecting minimal residual disease (MRD) in children with neuroblastoma (NB) by quantitative reverse transcriptase polymerase chain reaction (QRT-PCR) is not clear. This in part reflects the lack of uniform methodology for analysis and reporting. Reference laboratories across Europe have therefore established standard operating procedures (SOPs) for the detection of NB cells by QRT-PCR. Haemopoietic samples are collected into PAXgene™ blood RNA tubes, which stabilise mRNA for 48 h at room temperature and more than 6 months at -80 °C. Tyrosine hydroxylase (TH) was selected as the target for NB cell detection, expression is normalised to β2-microglobulin and reported using the ΔΔCt method. The sensitivity of QRT-PCR increased from 58% to 90% following the development of SOPs. A robust, transferable, objective method for the detection of NB cells by QRT-PCR has been defined to improve the power and consistency of studies on MRD in children with NB. © 2006 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
2007. Vol. 43, no 2, 341-350 p.
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Medical and Health Sciences
URN: urn:nbn:se:liu:diva-39397DOI: 10.1016/j.ejca.2006.08.007Local ID: 48230OAI: diva2:260246
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-11

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Kågedal, Bertil
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Faculty of Health SciencesDivision of clinical chemistryDepartment of Clinical Chemistry
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