Conjugated polymers as optical probes for protein interactions and protein conformations
2007 (English)In: Macromolecular rapid communications, ISSN 1022-1336, Vol. 28, no 17, 1703-1713 p.Article in journal (Refereed) Published
There is a need for highly sensitive, multi-parallel protein sensors within diagnostics and proteomic research. Conjugated polymers (CPs) have been demonstrated as highly sensitive optical probes for protein biosensing. Compared to small molecules, the polymeric probe has the possibility of multiple interactions and a collective response, which enhances the sensor signal. The optical output is colorimetric or, more sensitive, fluorescence based, including Förster energy transfer and changes in the emission wavelengths and/or intensity. Using CPs, many interesting protein detection events have been demonstrated, e.g., protein interactions, enzymatic activity, amyloid fibril formation, and detection by aptamers. CPs have also been successfully used to stain bacterial, cellular, and tissue samples. © 2007 WILEY-VCH Verlag GmbH & Co. KGaA.
Place, publisher, year, edition, pages
2007. Vol. 28, no 17, 1703-1713 p.
National CategoryNatural Sciences
IdentifiersURN: urn:nbn:se:liu:diva-39411DOI: 10.1002/marc.200700281Local ID: 48251OAI: oai:DiVA.org:liu-39411DiVA: diva2:260260