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Detection of molecular variants of prolactin in human serum, evaluation of a method based on ultrafiltration
Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Chemistry. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Medical and Health Sciences, Internal Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Heart and Medicine Center, Department of Endocrinology and Gastroenterology UHL.
Division of Clinical Chemistry, Kalmar County Hospital, Kalmar, Sweden.
Division of Clinical Chemistry, Kalmar County Hospital, Kalmar, Sweden.
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2007 (English)In: Clinica Chimica Acta, ISSN 0009-8981, E-ISSN 1873-3492, Vol. 376, no 1-2, 220-225 p.Article in journal (Refereed) Published
Abstract [en]

Background

In human blood, there are several molecular variants of prolactin with different biological effects. There is a need for new methods to detect and quantify these variants in order to fully understand the pathophysiological role of prolactin.

Methods

A method based on ultrafiltration was optimized, validated and compared to PEG precipitation. Serum samples from 84 patients were analyzed before and after pre treatment on two immunoassays, Elecsys (Roche) and Access (Beckman). Protein G precipitation was used to confirm presence of macroprolactin.

Results

The recovery of prolactin after ultrafiltration was lower than after PEG precipitation. A limit of 40% recovery after PEG precipitation corresponded to 27% recovery after ultrafiltration. Using these limits there were total agreement regarding detection of macroprolactin (rs = 0.96). In contrast, recovery of prolactin in samples without macroprolactin showed a considerable disagreement between ultrafiltration and PEG precipitation (rs = 0.48). Within-run CV was 4% for the ultrafiltration method. The correlation coefficient (r) between the immunoassays was 0.96 after ultrafiltration.

Conclusions

Ultrafiltration can be used to compare different prolactin immunoassays and to detect macroprolactin in assays with interference from PEG. For samples without macroprolactin ultrafiltration may give additional information reflecting individual variations of other molecular variants of prolactin.

Place, publisher, year, edition, pages
2007. Vol. 376, no 1-2, 220-225 p.
Keyword [en]
Prolactin, Macroprolactin, Ultrafiltration, PEG precipitation
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-39496DOI: 10.1016/j.cca.2006.08.029Local ID: 49048OAI: oai:DiVA.org:liu-39496DiVA: diva2:260345
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2014-02-14Bibliographically approved

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Landberg, EvaWahlberg Topp, JeanetteEkman, Bertil

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Department of Clinical ChemistryFaculty of Health SciencesInternal MedicineDepartment of Endocrinology and Gastroenterology UHLInternal Medicine
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