liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Detection of molecular variants of prolactin in human serum, evaluation of a method based on ultrafiltration
Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Clinical Chemistry. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Medical and Health Sciences, Internal Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Heart and Medicine Center, Department of Endocrinology and Gastroenterology UHL.
Division of Clinical Chemistry, Kalmar County Hospital, Kalmar, Sweden.
Division of Clinical Chemistry, Kalmar County Hospital, Kalmar, Sweden.
Show others and affiliations
2007 (English)In: Clinica Chimica Acta, ISSN 0009-8981, E-ISSN 1873-3492, Vol. 376, no 1-2, 220-225 p.Article in journal (Refereed) Published
Abstract [en]


In human blood, there are several molecular variants of prolactin with different biological effects. There is a need for new methods to detect and quantify these variants in order to fully understand the pathophysiological role of prolactin.


A method based on ultrafiltration was optimized, validated and compared to PEG precipitation. Serum samples from 84 patients were analyzed before and after pre treatment on two immunoassays, Elecsys (Roche) and Access (Beckman). Protein G precipitation was used to confirm presence of macroprolactin.


The recovery of prolactin after ultrafiltration was lower than after PEG precipitation. A limit of 40% recovery after PEG precipitation corresponded to 27% recovery after ultrafiltration. Using these limits there were total agreement regarding detection of macroprolactin (rs = 0.96). In contrast, recovery of prolactin in samples without macroprolactin showed a considerable disagreement between ultrafiltration and PEG precipitation (rs = 0.48). Within-run CV was 4% for the ultrafiltration method. The correlation coefficient (r) between the immunoassays was 0.96 after ultrafiltration.


Ultrafiltration can be used to compare different prolactin immunoassays and to detect macroprolactin in assays with interference from PEG. For samples without macroprolactin ultrafiltration may give additional information reflecting individual variations of other molecular variants of prolactin.

Place, publisher, year, edition, pages
2007. Vol. 376, no 1-2, 220-225 p.
Keyword [en]
Prolactin, Macroprolactin, Ultrafiltration, PEG precipitation
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-39496DOI: 10.1016/j.cca.2006.08.029Local ID: 49048OAI: diva2:260345
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2014-02-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Landberg, EvaWahlberg Topp, JeanetteEkman, Bertil
By organisation
Department of Clinical ChemistryFaculty of Health SciencesInternal MedicineDepartment of Endocrinology and Gastroenterology UHLInternal Medicine
In the same journal
Clinica Chimica Acta
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 65 hits
ReferencesLink to record
Permanent link

Direct link