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Simultaneous determination of sildenafil, its N-desmethyl metabolite and other drugs in human urine by gradient RP-HPLC method
Silesian University of Technology, Poland.
Silesian University of Technology, Poland.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Medicine and Care, Physiology. Östergötlands Läns Landsting, Heart Centre, Department of Clinical Physiology.
Silesian Centre of Heart Disease Medical University of Katowice.
2007 (English)In: Chemia Analityczna, ISSN 0009-2223, Vol. 52, no 4, 645-671 p.Article in journal (Refereed) Published
Abstract [en]

A new, rapid, sensitive and accurate gradient reversed-phase high-performance liquid chromatography technique for simultaneous separation and analysis of sildenafil citrate (SC), its N-desmethyl active metabolite - N-desmethylsildenafil (UK-103,320) in the presence of different drugs in human urine was developed. The analysed drugs were extracted from urine by liquid-liquid extraction. Effective RP-HPLC separation of the examined drugs was performed using a Merck LiChroCART® analytical column (Purospher® STAR RP-18 endcapped, 125 x 3 mm, particle size 5 μm) with a gradient mobile phase system and diode array or fluorescence detector. Linear ranges of detection for SC and UK-103,320 were found to be 0.03-8.5 μg mL-1 (r 2 = 0.9994) for both compounds. Linear ranges for other drugs (analgesic, antibiotic, diuretic and demulcent), which could exist in urine from patients treated with SC were also determined. Complete separation of all analytes was achieved below 25 min. The retention times for all studied analytes ranged from 4.76 to 18.84 min. The limits of detection and limits of quantification for both analysed compounds were calculated and recovery studies were also performed. The mean absolute recoveries of SC and UK-103,320 were > 94%. The new procedure was suitably validated and successfully applied for the analysis of SC, its active metabolite and other drugs in urine samples of patients with pulmonary hypertension.

Place, publisher, year, edition, pages
2007. Vol. 52, no 4, 645-671 p.
Keyword [en]
Sildenafil, N-desmethylsildenafil (UK-103, 320), drugs, human urine, RP-HPLC
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-40361Local ID: 53157OAI: diva2:261210
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-11

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