In vivo cytokine secretion and NF-κB activation around titanium and copper implants
2005 (English)In: Biomaterials, ISSN 0142-9612, Vol. 26, no 5, 519-527 p.Article in journal (Refereed) Published
The early biological response at titanium (Ti), copper (Cu)-coated Ti and sham sites was evaluated in an in vivo rat model. Material surface chemical and topographical properties were characterized using Auger electron spectroscopy, energy dispersive X-ray spectroscopy and interferometry, respectively. The number of leukocytes, cell types and cell viability (release of lactate dehydrogenase) were determined in the implant-interface exudate. The contents of activated nuclear transcription factor NF-κB, interleukin-6 (IL-6) and interleukin-10 (IL-10) were determined by enzyme linked immunosorbent assay. An increase in the number of leukocytes, in particular, polymorphonuclear leukocytes, was observed between 12 and 48h around Cu. A marked decrease of exudate cell viability was found around Cu after 48h. The total amounts of activated NF-κB after 12h was highest in Ti exudates whereas after 48h the highest amount of NF-κB was detected around Cu. The levels of cytokine IL-6 were consistently high around Cu at both time periods. No differences in IL-10 contents were detected, irrespective of material/sham and time. The results show that materials with different toxicity grades (titanium with low and copper with high toxicity) exhibit early differences in the activation of NF-κB, extracellular expression and secretion of mediators, causing major differences in inflammatory cell accumulation and death in vivo. © 2004 Elsevier Ltd. All rights reserved.
Place, publisher, year, edition, pages
2005. Vol. 26, no 5, 519-527 p.
IdentifiersURN: urn:nbn:se:liu:diva-41173DOI: 10.1016/j.biomaterials.2004.02.066Local ID: 55297OAI: oai:DiVA.org:liu-41173DiVA: diva2:262024