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Protein microarray analysis as a tool for monitoring cellular autoreactivity in type 1 diabetes patients and their relatives
Linköping University, Faculty of Health Sciences. Linköping University, Department of Molecular and Clinical Medicine, Pediatrics.
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2007 (English)In: Pediatric Diabetes, ISSN 1399-543X, Vol. 8, no 5, 252-260 p.Article in journal (Refereed) Published
Abstract [en]

Background: Autoreactive T cells have a crucial role in type 1 diabetes (T1D) pathogenesis.: Objectives: The aim of our study was to monitor the in vitro production of cytokines by peripheral blood mononuclear cells (PBMCs) after stimulation with diabetogenic autoantigens. Subjects: Ten T1D patients (tested at the time of diagnosis and 6 and 12 months later), 10 first-degree relatives of the T1D patients, and 10 controls underwent the study. Methods: PBMCs were stimulated with glutamic acid decarboxylase 65 (GAD65) amino acids (a.a.) 247-279, 509-528, and 524-543, proinsulin a.a. 9-23, and tyrosine phosphatase (islet antigen-2)/R2 a.a. 853-872. Interleukin (IL)-2, IL-4, IL-5, IL-6, IL-10, IL-13, interferon (IFN)-γ, tumor necrosis factor β, transforming growth factor β1, and granulocyte colony-stimulating factor (GCSF) were analyzed by protein microarray. Results: Differences in cytokine(s) poststimulatory and mainly in basal production were observed in all groups. The most prominent findings were in controls, the higher basal levels of IL-2, IL-4, IL-5, IL-13, and GCSF were observed when compared with relatives (p < 0.05, for all). After stimulation in controls, there was a significant decrease in IL-2, IL-13, GCSF, and IFN-γ (p < 0.05, for all). The group of relatives was the most variable in poststimulatory production. A strong correlation between cytokines production was found but groups differed in this aspect. Conclusion: By multiplex analysis, it may be possible, for example, to define the risk immunological response pattern among relatives or to monitor the immune response in patients on immune modulation therapy. © 2007 The Authors Journal compilation © 2007 Blackwell Munksgaard.

Place, publisher, year, edition, pages
2007. Vol. 8, no 5, 252-260 p.
Keyword [en]
autoantigen, autoreactive T cells, cytokine, protein microaray, T1D
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-41920DOI: 10.1111/j.1399-5448.2007.00308.xLocal ID: 59372OAI: oai:DiVA.org:liu-41920DiVA: diva2:262775
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-11

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Faresjö, Maria

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