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Phase memory relaxation times of spin labels in human carbonic anhydrase II: Pulsed EPR to determine spin label location
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biochemistry.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biochemistry.ORCID iD: 0000-0002-7642-9263
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2001 (English)In: Biophysical Chemistry, ISSN 0301-4622, E-ISSN 1873-4200, Vol. 94, no 3, 245-256 p.Article in journal (Refereed) Published
Abstract [en]

Phase memory relaxation times (TM or T2) of spin labels in human carbonic anhydrase II (HCA II) are reported. Spin labels (N-(1-oxyl-2,2,5,5-tetramethyl-3-pyrrolidinyl)iodoacetamide, IPSL) were introduced at cysteines, by site-directed mutagenesis at seven different positions in the protein. By two pulse electron paramagnetic resonance (EPR), electron spin echo decays at 45 K are measured and fitted by stretched exponentials, resulting in relaxation parameters TM and x. TM values of seven positions are between 1.6 ╡s for the most buried residue (L79C) and 4.7 ╡s for a residue at the protein surface (W245C). In deuteriated buffer, longer TM are found for all but the most buried residues (L79C and W97C), and electron spin echo envelop modulation (ESEEM) of deuterium nuclei is observed. Different deuterium ESEEM patterns for W95C and W16C (surface residue) indicate differences in the local water concentration, or accessibility, of the spin label by deuterium. We propose TM as a parameter to determine the spin label location in proteins. Furthermore, these systems are interesting for studying the pertaining relaxation mechanism. ⌐ 2001 Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
2001. Vol. 94, no 3, 245-256 p.
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Natural Sciences
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URN: urn:nbn:se:liu:diva-42075DOI: 10.1016/S0301-4622(01)00239-3Local ID: 60235OAI: oai:DiVA.org:liu-42075DiVA: diva2:262930
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13

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Hammarström, PerMårtensson, Lars-Göran

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