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Infiltration of forkhead box P3-expressing cells in small intestinal mucosa in coeliac disease but not in type 1 diabetes
Dept of Viral Diseases and Immunology National Public Health Inst, Helsinki, Finland.
Hospital for Children and Adolescents University of Helsinki, Finland.
Hospital for Children and Adolescents University of Helsinki, Finland.
Hospital for Children and Adolescents University of Helsinki, Finland.
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2008 (English)In: Clinical and Experimental Immunology, ISSN 0009-9104, E-ISSN 1365-2249, Vol. 152, no 3, 498-507 p.Article in journal (Refereed) Published
Abstract [en]

Because the role of regulatory T cells in the intestinal inflammation is unknown in coeliac disease (CD) and type 1 diabetes (T1D), the expression of forkhead box P3 (FoxP3), CD25, transforming growth factor-β, interferon (IFN)-γ, interleukin (IL)-4, IL-8, IL-10, IL-15 and IL-18 was measured by quantitative reverse transcription-polymerase chain reaction in the small intestinal biopsies from paediatric patients with active or potential CD, T1D and control patients. The numbers of FoxP3- and CD25-expressing cells were studied with immunohistochemistry. Enhanced intestinal expressions of FoxP3, IL-10 and IFN-γ mRNAs were found in active CD when compared with controls (P-values < 0.001, 0.004, <0.001). In potential CD, only the expression of IFN-γ mRNA was increased. The numbers of FoxP3-expressing cells were higher in active and potential CD (P < 0.001, P = 0.05), and the ratio of FoxP3 mRNA to the number of FoxP3-positive cells was decreased in potential CD when compared with controls (P = 0.007). The ratio of IFN-γ to FoxP3-specific mRNA was increased in active and potential CD (P = 0.001 and P = 0.002). Patients with T1D had no changes in regulatory T cell markers, but showed increased expression of IL-18 mRNA. The impaired up-regulation of FoxP3 transcripts despite the infiltration of FoxP3-positive cells in potential CD may contribute to the persistence of inflammation. The increased ratio of IFN-γ to FoxP3 mRNA in active and potential CD suggests an imbalance between regulatory and effector mechanisms. The increased intestinal expression of IL-18 mRNA in patients with T1D adds evidence in favour of the hypothesis that T1D is associated with derangements in the gut immune system. © 2008 The Author(s).

Place, publisher, year, edition, pages
2008. Vol. 152, no 3, 498-507 p.
Keyword [en]
coeliac disease, FoxP3, gut, regulatory T cells, type 1 diabetes
National Category
Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-42394DOI: 10.1111/j.1365-2249.2008.03662.xLocal ID: 63564OAI: oai:DiVA.org:liu-42394DiVA: diva2:263250
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13

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Vaarala, Outi

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