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Using the new Phadebas® Forensic Press test to find crime scene saliva stains suitable for DNA analysis
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology. Linköping University, Department of Physics, Chemistry and Biology, Molecular genetics .
2008 (English)In: Forensic Science International: Genetics Supplement Series, ISSN 1875-1768, Vol. 1, no 1, 430-432 p.Article in journal (Refereed) Published
Abstract [en]

The Phadebas® Forensic Press test is a new product that detects saliva stains by reacting with amylase. When the paper is pressed against a saliva stain a blue spot occurs. To test the sensitivity of the paper, a set of dilution series of saliva down to 1:500 was prepared on cotton fabric. Blue spots could be seen for dilutions of 1:100 when incubated at room temperature, and 1:200 in 37 °C. However, incubation at 37 °C did not provide acceptable reproducibility. The Phadebas® test was compared to four different lightsources for the ability to detect saliva on different carrier materials. Cotton fabric (T-shirt), denim, suede, leather, painted wood and untreated wood were tested. On denim, no stains could be detected with the lightsources, but Phadebas® showed all stains for pure saliva and dilution 1:5. DNA analysis was performed on detected stains and corresponding spots on the Phadebas® paper. Complete DNA profiles were produced for stains from pure saliva and dilution 1:5.

Place, publisher, year, edition, pages
2008. Vol. 1, no 1, 430-432 p.
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-42874DOI: 10.1016/j.fsigss.2007.10.205Local ID: 69679OAI: diva2:263731
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2010-09-24

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Ansell, Ricky
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The Institute of TechnologyDepartment of Physics, Chemistry and BiologyMolecular genetics
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