Using the new Phadebas® Forensic Press test to find crime scene saliva stains suitable for DNA analysis
2008 (English)In: Forensic Science International: Genetics Supplement Series, ISSN 1875-1768, Vol. 1, no 1, 430-432 p.Article in journal (Refereed) Published
The Phadebas® Forensic Press test is a new product that detects saliva stains by reacting with amylase. When the paper is pressed against a saliva stain a blue spot occurs. To test the sensitivity of the paper, a set of dilution series of saliva down to 1:500 was prepared on cotton fabric. Blue spots could be seen for dilutions of 1:100 when incubated at room temperature, and 1:200 in 37 °C. However, incubation at 37 °C did not provide acceptable reproducibility. The Phadebas® test was compared to four different lightsources for the ability to detect saliva on different carrier materials. Cotton fabric (T-shirt), denim, suede, leather, painted wood and untreated wood were tested. On denim, no stains could be detected with the lightsources, but Phadebas® showed all stains for pure saliva and dilution 1:5. DNA analysis was performed on detected stains and corresponding spots on the Phadebas® paper. Complete DNA profiles were produced for stains from pure saliva and dilution 1:5.
Place, publisher, year, edition, pages
2008. Vol. 1, no 1, 430-432 p.
National CategoryNatural Sciences
IdentifiersURN: urn:nbn:se:liu:diva-42874DOI: 10.1016/j.fsigss.2007.10.205Local ID: 69679OAI: oai:DiVA.org:liu-42874DiVA: diva2:263731