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Acute effects of insulin on the activity of mitochondrial GPAT1 in primary adipocytes
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Cell Biology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Cell Biology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Cell Biology.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Cell Biology.
2008 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 367, no 1, 201-207 p.Article in journal (Refereed) Published
Abstract [en]

The mitochondrial enzyme 1-acyl-sn-glycerol-3-phosphate acyltransferase (mtGPAT1) catalyzes a rate-limiting step in triacylglycerol and glycerophospholipid biosynthesis, which can be modulated by protein kinases in cell free analyses. We report that treatment of primary rat adipocytes with insulin acutely affects the activity of mtGPAT1 by increasing VMAX and KM for the substrates glycerol-3-phosphate and palmitoyl-CoA. Proteolytic cleavage of isolated mitochondrial membranes and mass spectrometric peptide sequencing identify in vivo phosphorylation of serine 632 and serine 639 in mtGPAT1. These phosphorylation sites correspond to casein kinase-2 consensus sequences and are highly conserved in chordate animal, but not fly, fungal or plant, mtGPAT1. © 2007 Elsevier Inc. All rights reserved.

Place, publisher, year, edition, pages
2008. Vol. 367, no 1, 201-207 p.
Keyword [en]
Adipocytes/*drug effects/metabolism Amino Acid Sequence Animals Binding Sites Casein Kinase II/metabolism Catalysis Glycerol-3-Phosphate O-Acyltransferase/*metabolism Glycerophospholipids/biosynthesis Insulin/*pharmacology Kinetics Mass Spectrometry Mitoc
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-43216DOI: 10.1016/j.bbrc.2007.12.127Local ID: 72980OAI: oai:DiVA.org:liu-43216DiVA: diva2:264075
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13

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Bronnikov, GennadyAboulaich, NabilaVener, AlexanderStrålfors, Peter

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