B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells
2008 (English)In: Blood, ISSN 0006-4971, E-ISSN 1528-0020, Vol. 112, no 4, 1048-1055 p.Article in journal (Refereed) Published
Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220 +CD19+ pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage- restricted IgII1 (λ5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN-B220- CD19-CD127 +FLT3+ SCA1lowKITlow population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B- lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19. © 2008 by The American Society of Hematology.
Place, publisher, year, edition, pages
2008. Vol. 112, no 4, 1048-1055 p.
Animals Antigens, CD19/*analysis Antigens, CD45/*analysis B-Lymphocytes/chemistry/*cytology Bone Marrow Cells *Cell Lineage Hematopoietic Stem Cells/chemistry/*cytology Humans Interleukin-2 Receptor alpha Subunit Mice Mice, Inbred C57BL Mice, Transgenic
National CategoryMedical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-43397DOI: 10.1182/blood-2007-11-125385Local ID: 73745OAI: oai:DiVA.org:liu-43397DiVA: diva2:264256