liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Vasopressin receptor mRNA expression in the human gastrointestinal tract
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Clinical Microbiology . Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Molecular Biological Techniques.
Department of Clinical Sciences Malmö University.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine.
Department of Clinical Sciences Malmö University.
2008 (English)In: European Surgical Research, ISSN 0014-312X, E-ISSN 1421-9921, Vol. 40, no 1, 34-40 p.Article in journal (Refereed) Published
Abstract [en]

Background/Aim: Vasopressin and oxytocin are closely related peptides, and both exert effects on the gastrointestinal function. In the present study, we wanted to map the expression of vasopressin receptor mRNAs (V1a, V1b/V3, and V2) in nontumorous tissue biopsy specimens of human gastrointestinal tract and surrounding tissues. Methods: Total and polyA+ RNAs were isolated from human tissue biopsy specimens using an automated nucleic acid extractor and, subsequently, converted into single-stranded cDNA. Seminested PCR amplifications were carried out, using gene-specific V1a, V1b/V3, and V2 receptor primers. The PCR amplicons were partially sequenced to confirm their identity. Results: The present study demonstrated the expression of vasopressin receptor mRNAs in human gastrointestinal tract, pancreas, kidney, lung, brain, and ovary. The expression pattern varied between different parts of the gastrointestinal tract. In the colon ascendens, V1a receptor mRNA expression could not be detected in 3 out of 4 analyzed tissue biopsy specimens. On the other hand, all the vasopressin receptor mRNAs were expressed in all colon transversum biopsy samples. Conclusions: V1a, V1b/V3, and V2 receptor mRNAs are widely expressed throughout human gastrointestinal tract and surrounding tissues. The data obtained provide information for further mapping and determination of the physiological role of the vasopressin receptor mRNA expression in normal and tumorous tissues.

Place, publisher, year, edition, pages
2008. Vol. 40, no 1, 34-40 p.
Keyword [en]
Aged Aged, 80 and over Female Gastrointestinal Tract/*physiology Gene Expression/physiology Humans Male Middle Aged RNA, Messenger/metabolism Receptors, Vasopressin/*genetics Reverse Transcriptase Polymerase Chain Reaction
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-43400DOI: 10.1159/000108655Local ID: 73751OAI: oai:DiVA.org:liu-43400DiVA: diva2:264259
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13

Open Access in DiVA

No full text

Other links

Publisher's full texthttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17890865

Authority records BETA

Monstein, Hans-JurgRyberg, Anna

Search in DiVA

By author/editor
Monstein, Hans-JurgRyberg, Anna
By organisation
Faculty of Health SciencesClinical Microbiology Department of Molecular Biological TechniquesDepartment of Clinical and Experimental Medicine
In the same journal
European Surgical Research
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 70 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf