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Design of a multiplex nested PCR for genotyping of the NSP4 from group A rotavirus
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine.
Swedish Institute for Infectious Disease Control Solna.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine.
Swedish Institute for Infectious Disease Control Solna.
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2008 (English)In: Journal of Virological Methods, ISSN 0166-0934, E-ISSN 1879-0984, Vol. 149, no 2, 240-245 p.Article in journal (Refereed) Published
Abstract [en]

A novel PCR method was developed to discriminate amongst genotypes A-C of the rotavirus non-structural protein 4 (NSP4). Genotype-specific primers were designed that correctly identified the NSP4 genotype when evaluated as a multiplex PCR with cell culture adapted rotavirus strains. Rotavirus strains B223 SGIG6P6[1], NCDV SGIG6P6[1] and SA11 SGIG3P5B[2] were used as control for NSP4 genotype A, A34 SGIG5P14[23], Gottfried SGIIG4P2B[6] and Wa SGIIG1P1A[8] for NSP4 genotype B, RRV SGIG3P5B[3] for NSP4 genotype C. Subsequently, the same set of specific primers was used to genotype a set of 77 Swedish clinical samples. The results showed that all human clinical samples analyzed belong to the NSP4 genotype B and the VP6 subgroup II. © 2008 Elsevier B.V. All rights reserved.

Place, publisher, year, edition, pages
2008. Vol. 149, no 2, 240-245 p.
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Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-43457DOI: 10.1016/j.jviromet.2008.01.030Local ID: 73896OAI: oai:DiVA.org:liu-43457DiVA: diva2:264316
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13

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Rodriguez, JesusSpitzner, MarkusSvensson, Lennart

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