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Clinical impact of real-time evaluation of the biological activity and degradation of hepatocyte growth factor
Linköping University, Department of Clinical and Experimental Medicine, Infectious Diseases. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Infectious Diseases. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Physics, Chemistry and Biology. Linköping University, The Institute of Technology.ORCID iD: 0000-0002-7001-9415
Linköping University, Department of Medical and Health Sciences. Linköping University, Faculty of Health Sciences.
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2008 (English)In: Growth Factors, ISSN 0897-7194, E-ISSN 1029-2292, Vol. 26, no 3, 163-171 p.Article in journal (Refereed) Published
Abstract [en]

Hepatocyte growth factor (HGF) is essential for injury repair. Despite high HGF levels in chronic ulcers, up-regulation of HGF receptor in ulcer tissue and decreased biological activity of HGF in ulcer secretions have been observed. With a surface plasmon resonance-based method, we assessed the binding of HGF to antibodies, receptors, and the basement membrane and identified binding interactions that are indispensable for the biological activity of HGF. Recombinant HGF (rHGF) lots were tested for activity, structural integrity, and degradation, and the results were verified in an in vitro model of cell injury. Biologically active rHGF, as well as plasma from healthy volunteers, bound to heparan sulphate proteoglycan (HSPG) and to anti-HGF antibodies. Decreased binding to HSPG was the first event in rHGF degradation. This study established the feasibility of identifying patients with chronic inflammation who need exogenous HGF and of using ligand-binding assessment to evaluate rHGF lots for biological activity.

Place, publisher, year, edition, pages
2008. Vol. 26, no 3, 163-171 p.
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Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-43460DOI: 10.1080/08977190802128083Local ID: 73902OAI: oai:DiVA.org:liu-43460DiVA: diva2:264319
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2017-12-13Bibliographically approved

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Nayeri, FaribaAili, DanielBrudin, LarsLiedberg, Bo

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Nayeri, FaribaAili, DanielBrudin, LarsLiedberg, Bo
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Infectious DiseasesFaculty of Health SciencesDepartment of Physics, Chemistry and BiologyThe Institute of TechnologyDepartment of Medical and Health Sciences
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Growth Factors
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