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Recombinant human collagen for tissue engineered corneal substitutes
Deptartment of Cellular and Molecular Medicine University of Ottawa, Ottawa, Ontario, Canada.
University of Ottawa Eye Institute, 501 Smyth Road, Ottawa, Ontario K1H8L6, Canada.
Deptartment of Cellular and Molecular Medicine University of Ottawa, Ottawa, Ontario, Canada.ORCID iD: 0000-0003-1222-6720
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Ophthalmology. Östergötlands Läns Landsting, Reconstruction Centre, Department of Ophthalmology UHL.
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2008 (English)In: Biomaterials, ISSN 0142-9612, E-ISSN 1878-5905, Vol. 29, no 9, 1147-1158 p.Article in journal (Refereed) Published
Abstract [en]

We successfully fabricated transparent, robust hydrogels as corneal substitutes from concentrated recombinant human type I and type III collagen solutions crosslinked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS). White light transmission through these gels is comparable or superior to that of human corneas. Hydrogels from both type I and type III collagens supported in vitro epithelium and nerve over-growth. While both these biocompatible hydrogels have adequate tensile strength and elasticity for surgical manipulation, type III collagen hydrogels tended to be mechanically superior. Twelve-month post-implantation results of type I recombinant collagen-based corneal substitutes into mini-pigs showed retention of optical clarity, along with regeneration of corneal cells, nerves and tear film. For clinical use, implants based on fully characterized, recombinant human collagen eliminate the risk of pathogen transfer or xenogeneic immuno-responses posed by animal collagens. © 2007 Elsevier Ltd. All rights reserved.

Place, publisher, year, edition, pages
2008. Vol. 29, no 9, 1147-1158 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-43660DOI: 10.1016/j.biomaterials.2007.11.011Local ID: 74496OAI: diva2:264520
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2013-12-17

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Griffith, MayFagerholm, PerLagali, Neil
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Faculty of Health SciencesOphthalmologyDepartment of Ophthalmology UHL
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