Analysis of carbohydrates using liquid chromatography-surface plasmon resonance immunosensing systems
2000 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 281, no 2, 151-158 p.Article in journal (Refereed) Published
An immunosensing system based on surface plasmon resonance (SPR) was used for on-line detection and characterization of carbohydrate molecules separated by high-performance liquid chromatography. These analytes, with or without serum, were continuously separated and analyzed in the combined liquid chromatography-surface plasmon resonance (LC-SPR) system. By using weak and readily reversible monoclonal antibodies, the SPR system allowed specific on-line monitoring of the substances. To increase the specificity of the immunosensor, nonrelevant antibodies were used as reference in a serial flow cell. The sensitivity of the LC-SPR system was dependent on molecular weight of the carbohydrate, affinity of binding, and design of the sensor. (C) 2000 Academic Press.
Place, publisher, year, edition, pages
2000. Vol. 281, no 2, 151-158 p.
National CategoryNatural Sciences
IdentifiersURN: urn:nbn:se:liu:diva-43746DOI: 10.1006/abio.2000.4565Local ID: 74654OAI: oai:DiVA.org:liu-43746DiVA: diva2:264606