liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Differential roles of PDGFR-(alpha) and PDGFR-(beta) in angiogenesis and vessel stability
Karolinska Institute.
Karolinska Institute.
Shandong University.
Shandong University.
Show others and affiliations
2009 (English)In: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 23, 153-163 p.Article in journal (Refereed) Published
Abstract [en]

Preclinical and clinical evaluations of individual proangiogenic/arteriogenic factors for the treatment of ischemic myocardium and skeletal muscle have produced unfulfilled promises. The establishment of functional and stable arterial vascular networks may require combinations of different angiogenic and arteriogenic factors. Using in vivo angiogenesis and ischemic hind-limb animal models, we have compared the angiogenic and therapeutic activities of fibroblast growth factor 2 (FGF-2) in combinations with PDGF-AA and PDGF-AB, two members of the platelet-derived growth factor (PDGF) family, with distinct receptor binding patterns. We show that both PDGF-AA/FGF-2 and PDGF-AB/FGF-2 in combinations synergistically induce angiogenesis in the mouse cornea. FGF-2 up-regulates PDGFR- and -β expression levels in the newly formed blood vessels. Interestingly, PDGF-AB/FGF-2, but not PDGF-AA/FGF-2, is able to stabilize the newly formed vasculature by recruiting pericytes, and an anti-PDGFR-β neutralizing antibody significantly blocks PDGF-AB/FGF-2-induced vessel stability. These findings demonstrate that PDGFR-β receptor is essential for vascular stability. Similarly, PDGF-AB/FGF-2 significantly induces stable collateral growth in the rat ischemic hind limb. The high number of collaterals induced by PDGF-AB/FGF-2 leads to dramatic improvement of the paw’s skin perfusion. Immunohistochemical analysis of the treated skeletal muscles confirms that a combination of PDGF-AB and FGF-2 significantly induces arteriogenesis in the ischemic tissue. A combination of PDGF-AB and FGF-2 would be optimal proangiogenic agents for the treatment of ischemic diseases.—Zhang, J., Cao, R., Zhang, Y., Jia, T., Cao, Y., Wahlberg, E. Differential roles of PDGFR- and PDGFR-β in angiogenesis and vessel stability.

Place, publisher, year, edition, pages
2009. Vol. 23, 153-163 p.
Keyword [en]
neovascularization • ischemia • growth factor • receptor
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-43894DOI: 10.1096/fj.08-113860Local ID: 75046OAI: diva2:264755
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2010-08-20

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Zhang, JunhangWahlberg, Eric
In the same journal
The FASEB Journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 62 hits
ReferencesLink to record
Permanent link

Direct link