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Electronic nose for estimation of product concentration in mammalian cell cultivation
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biotechnology .
2000 (English)In: Bioprocess engineering (Berlin. Print), ISSN 0178-515X, Vol. 23, no 6, 637-642 p.Article in journal (Refereed) Published
Abstract [en]

The off-gas composition from perfusion cultivation of a CHO-cell line producing recombinant human blood coagulation Factor VIII is monitored with an electronic nose. It is shown that the electronic nose in combination with an artificial neural network can be used for on-line estimation of the Factor VIII concentration in production-scale cultivations. The obtained prediction error (1s) for the Factor VIII concentration was 1.1 IU/ml. The potential of the electronic nose for estimation of viable cell count is outlined in laboratory-scale Factor VIII cultivations. The obtained prediction error (1s) for the viable cell count was 0.4 ╫ 106 cells/ml. The results show that this non-invasive method is potentially useful for on-line bioprocess monitoring.

Place, publisher, year, edition, pages
2000. Vol. 23, no 6, 637-642 p.
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-44219DOI: 10.1007/s004490000213Local ID: 76075OAI: diva2:265080
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-14

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Mandenius, Carl-Fredrik
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