liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Electronic nose for estimation of product concentration in mammalian cell cultivation
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biotechnology .
2000 (English)In: Bioprocess engineering (Berlin. Print), ISSN 0178-515X, Vol. 23, no 6, 637-642 p.Article in journal (Refereed) Published
Abstract [en]

The off-gas composition from perfusion cultivation of a CHO-cell line producing recombinant human blood coagulation Factor VIII is monitored with an electronic nose. It is shown that the electronic nose in combination with an artificial neural network can be used for on-line estimation of the Factor VIII concentration in production-scale cultivations. The obtained prediction error (1s) for the Factor VIII concentration was 1.1 IU/ml. The potential of the electronic nose for estimation of viable cell count is outlined in laboratory-scale Factor VIII cultivations. The obtained prediction error (1s) for the viable cell count was 0.4 ╫ 106 cells/ml. The results show that this non-invasive method is potentially useful for on-line bioprocess monitoring.

Place, publisher, year, edition, pages
2000. Vol. 23, no 6, 637-642 p.
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-44219DOI: 10.1007/s004490000213Local ID: 76075OAI: diva2:265080
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-14

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Mandenius, Carl-Fredrik
By organisation
The Institute of TechnologyBiotechnology
In the same journal
Bioprocess engineering (Berlin. Print)
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 15 hits
ReferencesLink to record
Permanent link

Direct link