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Neoglycoconjugates as affinity ligands in surface plasmon resonance analysis
Linköping University, Department of Physics, Chemistry and Biology, Biotechnology. Linköping University, Faculty of Science & Engineering.
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biotechnology.
2001 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 449, no 1-2, 51-58 p.Article in journal (Refereed) Published
Abstract [en]

Neoglycoconjugates, i.e. de novo synthesized conjugates of glycosides and carrier molecules such as proteins or polylipids, were used as affinity ligands in a surface plasmon resonance (SPR) biosensor assay. The affinity of the glycoconjugates, normally in the lower affinity range, was influenced by increasing the number of glycosides bound per carrier protein, the coupling chemistry, and the assay temperature. As a model system, albumin-conjugated A- and B-active blood group oligosaccharides with suitable linker molecules were chosen. Specific monoclonal antibodies were used as analytes to verify the interactive performance. Affinity kinetics of the systems were evaluated from the SPR biosensor data. The results demonstrate that neoglycoconjugates are a good alternative in presenting affinity ligands of small, specific molecules in SPR-based assays with retained specificity and bioavailability. ⌐ 2001 Elsevier Science B.V. All rights reserved.

Place, publisher, year, edition, pages
2001. Vol. 449, no 1-2, 51-58 p.
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-44235DOI: 10.1016/S0003-2670(01)01393-9Local ID: 76096OAI: diva2:265096
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2015-09-18

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Jungar, ChristinaMandenius, Carl-Fredrik
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