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Assessment of the performance of a fed-batch cultivation from the preculture quality using an electronic nose
Linköping University, The Institute of Technology. Linköping University, Department of Physics, Chemistry and Biology, Biotechnology .
2002 (English)In: Biotechnology progress (Print), ISSN 8756-7938, Vol. 18, no 2, 380-386 p.Article in journal (Refereed) Published
Abstract [en]

An electronic nose, a gas-phase multisensor system, was used to monitor precultivations of a recombinant tryptophan-producing Escherichia coli strain. The electronic nose signals showed a high correlation toward the main stages of the precultivations, namely, exponential growth, oxygen-limited growth, and glucose depletion. Principal component analysis (PCA) of the electronic nose signals was performed and shown to be useful for monitoring preculture progression. More importantly, PCA also allowed a qualitative assessment of the preculture performance during subsequent fed-batch cultivations. The electronic nose signals from the precultures showed, furthermore, a high correlation to the time of phosphate limitation and the tryptophan yield coefficient of the subsequent fed-batch cultivations, which allowed an accurate prediction of these process variables using partial least squares (PLS). The results demonstrate on data from 12 cultivations how the electronic nose can be a useful tool for the assessment of inoculum quality, thereby providing means of reducing batch-to-batch variation and increasing the productivity of bioprocesses.

Place, publisher, year, edition, pages
2002. Vol. 18, no 2, 380-386 p.
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-44243DOI: 10.1021/bp010166jLocal ID: 76106OAI: diva2:265105
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2011-01-13

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Mandenius, Carl-Fredrik
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