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Characterisation of a new degradable polymer scaffold for regeneration of the dermis: In vitro and in vivo human studies
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Burn Center. Östergötlands Läns Landsting, Reconstruction Centre, Department of Plastic Surgery, Hand surgery UHL.
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2008 (English)In: Organogenesis, ISSN 1547-6278, Vol. 4, no 3, 195-200 p.Article in journal (Refereed) Published
Abstract [en]

Full thickness skin wounds in humans heal with scars, but without regeneration of the dermis. A degradable poly(urethane urea) scaffold (PUUR), Artelon® is already used to reinforce soft tissues in orthopaedics, and for treatment of osteoarthritis of the hand, wrist and foot. In this paper we have done in vitro experiments followed by in vivo studies to find out whether the PUUR is biocompatible and usable as a template for dermal regeneration. Human dermal fibroblasts were cultured on discs of PUUR, with different macrostructures (fibrous and porous). They adhered to and migrated into the scaffolds, and produced collagen. The porous scaffold was judged more suitable for clinical applications and 4 mm Ø, 2 mm-thick discs of porous scaffold (12% w/w or 9% w/w polymer solution) were inserted intradermally in four healthy human volunteers. The implants were well tolerated and increasing ingrowth of fibroblasts was seen over time in all subjects. The fibroblasts stained immunohistochemically for procollagen and von Willebrand factor, indicating neocollagenesis and angiogenesis within the scaffolds. The PUUR scaffold may be a suitable material to use as a template for dermal regeneration. ©2008 Landes Bioscience.

Place, publisher, year, edition, pages
2008. Vol. 4, no 3, 195-200 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-44440Local ID: 76642OAI: diva2:265302
Available from: 2009-10-10 Created: 2009-10-10 Last updated: 2015-03-17Bibliographically approved
In thesis
1. Guided Regeneration of the Human Skin: in vitro and in vivo studies
Open this publication in new window or tab >>Guided Regeneration of the Human Skin: in vitro and in vivo studies
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Every day and in all parts of the world, humans experience different grades of wounding and tissue loss of the skin, thus initiating one of the most complex biological processes. Acute and chronic wounds, as well as the additional problem of skin scarring, involve not only great suffering for the patient but also extensive health care costs for the society. Although the wound-healing process is a wellstudied field much knowledge must be gained to unlock the door to regenerative pathways in humans.

Epidermis heals by complete regeneration, but dermal and full thickness injuries heal with fibrosis and scar formation. In Papers I and II, we studied whether dermal scarring could be turned into regeneration by using two different types of threedimensional dermal scaffolds. In Paper I, we studied a solid scaffold made of poly(urethane urea), initially in vitro then followed by in vivo studies. In Paper II, we intradermally injected a liquid three-dimensional scaffold consisting of porous gelatin spheres in human healthy volunteers. Both materials showed ingrowth of functional fibroblasts and blood vessels and appeared to stimulate regeneration while slowly degrading. This finding could be of significant clinical importance, for example in burn wound care or after cancer surgery.

In Papers III and IV, we wanted to study the effects of amniotic fluid and hyaluronic acid on adult wound healing, because early fetal wounds re-epithelialize rapidly and naturally heal dermis by regeneration without the need of a dermal scaffold. Amniotic fluid, naturally rich in hyaluronic acid, induced an accelerated reepithelialization of adult human wounds in vitro, and hyaluronic acid seemed to be important for this effect. Stimulation with exogenous hyaluronic acid in vivo induced accelerated re-epithelialization and an altered protein expression in healthy human volunteers. The inflammatory phase of wound healing, as measured by tissue viability imaging, was not affected by hyaluronic acid. Elucidating the effects of amniotic fluid and hyaluronic acid on the wound-healing process may allow improved treatment of wounds with impaired healing.

Studies on finding new dermal scaffolds and studies on the positive effect of amniotic fluid or hyaluronic acid on the wound-healing process are two different ways of gaining insight that may lead to regeneration and improved wound healing for the patient.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2015. 95 p.
Linköping University Medical Dissertations, ISSN 0345-0082 ; 1450
National Category
Medical Bioscience Cell Biology
urn:nbn:se:liu:diva-115598 (URN)10.3384/diss.diva-115598 (DOI)978-91-7519-114-0 (print) (ISBN)
Public defence
2015-04-20, Berzeliussalen, Campus US, Linköping, 09:00 (Swedish)
Available from: 2015-03-17 Created: 2015-03-17 Last updated: 2015-03-17Bibliographically approved

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Huss, FredrikKratz, Gunnar
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Faculty of Health SciencesBurn CenterDepartment of Plastic Surgery, Hand surgery UHLPlastic Surgery, Hand Surgery and Burns
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