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Effect of human milk on type 1 and P-fimbrial mRNA expression in intestinal Escherichia coli strains
Department of Clinical Bacteriology, Göteborg University, Göteborg, Sweden, Department of Clinical Bacteriology, Göteborg University, Guldhedsgatan 10, S-413 46 Göteborg, Sweden.
Linköping University, Faculty of Health Sciences. Linköping University, Department of Clinical and Experimental Medicine, Clinical Microbiology . Östergötlands Läns Landsting, Centre for Laboratory Medicine, Department of Molecular Biological Techniques.
Department of Clinical Bacteriology, Göteborg University, Göteborg, Sweden.
Department of Clinical Bacteriology, Göteborg University, Göteborg, Sweden.
2005 (English)In: Letters in Applied Microbiology, ISSN 0266-8254, E-ISSN 1472-765X, Vol. 40, no 1, 74-80 p.Article in journal (Refereed) Published
Abstract [en]

Aims: Escherichia coli from breastfed infants express more type 1 fimbriae and less P fimbriae than E. coli from bottle-fed infants. In this study we investigated the effect of human milk on production of mRNA for fimA (type 1 fimbriae) and papC (P fimbriae) in E. coli. Methods and Results: Production of adhesin gene mRNA was estimated using a reverse transcriptase polymerase chain reaction in E. coli strains under different culture conditions. More type 1 fimbrial mRNA was produced after culture in human milk (P = 0.001) or Luria broth (P = 0.014) than after culture on agar, whereas P-fimbrial mRNA production was similar under all tested growth conditions. When cultured on agar, E. coli strains carrying both the fim and pap operons produced less type 1 and P-fimbrial mRNA than strains that had only the fim or pap operons, respectively (P = 0.03 and 0.056). Significance and Impact of the Study: Environmental regulation of adhesin expression may be influenced by cross-talk between fimbrial operons.

Place, publisher, year, edition, pages
2005. Vol. 40, no 1, 74-80 p.
Keyword [en]
Bacterial adhesins, Bacterial RT-PCR, cDNA, Fimbriae, Gene expression, Virulence factors
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-45509DOI: 10.1111/j.1472-765X.2004.01631.xOAI: oai:DiVA.org:liu-45509DiVA: diva2:266405
Available from: 2009-10-11 Created: 2009-10-11 Last updated: 2017-12-13

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Monstein, Hans-Jurg

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